Gene Expression is the process by which information from a gene is used to direct the synthesis of a functional gene product. These products are often proteins. Gene expression analysis is critical to understand the functions of genes and its protein products, as well as to get a clearer picture of the complex regulatory networks that effect fundamental biological processes.

Signosis has a number of assay products for the gene expression study, including the cDNA Plate Arrays for monitoring the expression of genes that are up or down regulated by TFs, and the single cell PCR kits for analyzing gene expression, which allows direct analysis of cell lysates without RNA isolation prior assay.

 

Single Cell Gene Expression Analysis

With the Single Cell Real-Time RT-PCR or Single Cell RT-PCR kit, gene expression can be analyzed from cell lysates directly without the need of RNA preparation.

 

Product Name Catalog # Price   Qty
Direct cDNA Cell Lysis Buffer CL-0001 $377
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Single Cell RT-PCR Assay Kit CL-0002 $492
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Single Cell Real-Time RT-PCR Assay Kit CL-0003 $557
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Northern Blot Assay

Northern Blot is a standard method for detection and quantitation of mRNA expression levels. It allows visualization of intact mRNA and provides a direct relative comparison of mRNA abundance between samples on a single membrane. The assay kits are non-radioactive with all reagents included, and the probes are biotin pre-labeled. 

Benefits:
  • No Isotope required - Safe method of detection using chemiluminescence.
  • All reagents in one kit- Everything needed to perform the assay is included.
  • Simple procedure - No RNA isolation required, total RNA can be used directly.
  • High SNR - With optimized hybridization buffer and hybridization wash buffer, SNR( Signoal-Noise-Ratio) is good with low background.
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cDNA Plate Arrays from RNA

Each of the plate-based arrays can be used for profiling more than 20 genes.

Benefits:
  • Simple - Gene expression profiling is a easy as performing ELISA.
  • Highly sensitive detection - The cDNAs used to detect mRNA molecules contain multiple biotin labels for enhanced detection.

  • Quantitative comparison - The gene expression of 20+ genes in two or more samples can be quantitatively compared in one assay.
Product Name Catalog # Price   Qty
Human NFκB-Regulated cDNA Plate Array AP-0101 $648
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Human HIF-Regulated cDNA Plate Array AP-0111 $648
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Human Apoptosis cDNA Plate Array I AP-0121 $648
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Human Mitochondria Apoptosis cDNA Plate Array AP-0131 $648
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Human p53-Regulated cDNA Plate Array AP-0141 $648
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Human Stat3-Regulated cDNA Plate Array AP-0151 $648
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Human Akt Pathway cDNA Plate Array AP-0161 $648
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Human Wnt/β-Catenin Regulated cDNA Plate Array AP-0171 $648
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Human Stem Cell cDNA Marker Plate Array AP-0181 $648
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Human T Cell Receptor Signaling cDNA Plate Array AP-0191 $648
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Human PPAR Regulated cDNA Plate Array AP-0201 $648
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Human P450 cDNA Plate Array AP-0202 $648
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Human Toxicity cDNA Plate Array AP-0203 $648
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Human Cytokine cDNA Plate Array AP-0204 $648
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Mouse NFκB-Regulated cDNA Plate Array AP-1101 $648
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Mouse HIF-Regulated cDNA Plate Array AP-1111 $648
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Rat HIF-regulated cDNA Plate Array AP-1121 $648
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Mouse Inflammation cDNA Plate Array AP-1131 $648
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Mouse Cytokine cDNA Plate Array AP-1141 $648
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Rat PPAR gamma Regulated cDNA Plate Array AP-1161 $648
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Mouse Wnt/b-Catenin Regulated cDNA Plate Array AP-1171 $648
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Rat P450 cDNA plate Array AP-1181 $648
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cDNA Plate Arrays from Cell Lysates

Each of the plate-based arrays can be used for profiling the expression of more than 20 genes directly from cell lysates without the need of RNA preparation.
Benefits:
  • Simple - DNA can be synthesized  directly in cell lysates without RNA preparation.  In addition, the detection is as simple as ELISA.
  • Quantitative comparison - Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.
  • Quantitative comparison  - The difference of two or more samples in gene expression can be quantitatively analyzed. 
Product Name Catalog # Price   Qty
Human NFκB-Regulated cDNA Profiling in Cell Lysates AP-3101 $635
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Human HIF-Regulated cDNA Profiling in Cell Lysates AP-3102 $635
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