Transcription factors (TFs) are DNA binding proteins that play essential roles in regulating gene expression. They act as sensors to monitor changes of cells and convert the signals into changes of gene expression. Often, a specific cellular signal pathway can activate multiple TFs and the expression of a specific gene is under the control of multiple TFs.

Signosis provides a variety of tools to meet a variety of needs and facilitate the analysis of transcription factors. The TF Activation Profiling Plate Arrays, Transcriptional Interaction TF Plate Arrays, and Promoter-Binding TF Profiling Array are for the analysis of multiple TFs, and TF Filter Plate Assays, EMSA Kits, Luciferase Reporter Vectors and Vector Sets, Luciferase Stable Cell Lines, TF ELISA, and TF Western Blot Detection Assay Kits can monitor individual TFs.

  

TF Activation Profiling Plate Arrays

Profile the activation for up to 96 TFs at one time.

Benefits:

• Multiplex - One assay allows the measurement of the activities for various transcription factors.
• Quantitative comparison - Differences between samples can be quantitatively compared and analyzed.
• Simple procedures - Use simple procedures similar to ELISA.
• No special requirement - Use standard laboratory instrument, Luminex is not  needed.
• Works with Mammalian Nuclear Extracts-  Samples can be taken from human, mouse, or rat and more.

TF Promoter-Binding Profiling Plate Arrays

Determine whether 48 or 96 TFs bind to a promoter or not.

Benefits:

Multiplex - A single assay allows the characterization of binding of 48 or 96 TFs to a specific promoter. Simple procedures - Probe incubation, spin column separation, plate hybridization, and HRP detection.

TF Interaction Plate Arrays

Monitor TF/TF or TF/coregulator interactions among 48 or 96 different TFs simultaneously in relation to one TF or coregulator of interest.

Benefits:

Multiplex - One assay allows the measurement of the transcriptional interaction of 48 or 96 TFs with one specific TF or coregulator of interest. Quantitative comparison - Differences between samples can be quantitatively compared and analyzed. Simple procedures - Co-IP of TF complexes with magnetic beads is quick, easy, and effective. No special equipment required - Compatible with common chemiluminescence plate readers.

EMSA

100+ non-radioactive EMSA Kits are available. The probes are pre-labeled with biotin, and all reagents are included in the kit. 

Benefits:

• Isotope free - It is a chemiluminescent-based detection.
• No probe preparation - The probes included are pre-labeled and optimized.
• Highly Sensitive - With optimized conditions, the kit sensitivity is compatible with that from radioactive-based detection method.
• Simple procedure - All of the reagents are included in the kit.

 

FewCell TF Activation Profiling Plate Arrays

Profile the activation of TFs with limited amount of cells.

Benefits:

Designed for precious and limited cells - Requires as few as 2,000 cells. Multiplex - One assay allows the measurement of the activities for 16 transcription factors. One-step sample preparation - Obtain whole cell lysate with provided buffer.  Quantitative comparison - Differences between samples can be quantitatively compared and analyzed. Simple procedures - Use simple procedures similar to ELISA. No special requirement - Use standard laboratory instrument, Luminex is not needed.

TF Filter Plate Assays

This "EMSA on a plate" assay allows you to monitor the activation of a TF in vitro in up to 96 samples at one time. Benefits:

High throughput screen - A specific TF in multiple samples can be analyzed. Flexible - Can analyze 1 to 96 samples simultaneously. Easy to reseal and store for later use. Quantitative comparison - TF activity differences between two or more samples can be quantitatively analyzed. Wide selection - 100+ popular TFs Kits are available from Signosis.

TF Luciferase Reporter Vectors

Measure TF activation in cells. 100+ vectors are available. We also provide custom service to create a vector for any unlisted TFs.

pTF-Luc reporter vectors are a series of firefly luciferase-based reporter constructs for quantitative measurement of the activities of transcription factors (TFs) in cells. Each vector contains a cis-element (DNA binding sequence), a minimal promoter, and firefly luciferase gene. When a TF is activated, it binds to cis-element and results in induction of luciferase gene expression. Therefore, luciferase activity represents the activity of the TF.

Benefits:

• Functional assay - Monitor the transactivation of transcription factors in cells.
• Quantitative analysis - Determine TF activation by quantitative measurement ofluciferase activity.
• Stable cell lines - The vectors can be used for establishing stable cells expressing the reporters.  

Firefly Luciferase Substrate  for use with the reporter vectors.

QuantiTF Transcription Factor Activation ELISA Kits

Quantitatively analyze a specific transcription factor binding activity with the corresponding active recombinant protein as a reference standard 

Benefits:

• Quantitative - Active recombinant protein is provided as a standard corresponding to DNA binding activity level
• Functional assay – Detect transcription factor activation, not only expression. 
• Specific –Identify a particular gene member with an antibody against the specific TF protein.
• Sensitive – Optimized conditions with highest signal/noise ratio. 
• All-in-one – All reagents required for the assay are included. 
• Straightforward and simple procedure – Can be finished in 3 hours with minimal hands-on time.

 

 

TF ELISA Kits

TF ELISA Kit allows High-throughput profiling TF-DNA binding activity. Benefits:

Positive protein control included- The kit contains an active recombinant protein for a positive control. Highly effective - Distinguishing two pathways in one ELISA assay. Highly specific - Specifically monitor TF activation both the DNA binding sequence and gene specific antibody against the specific gene. High-throughput - With Whole Cell Lysis Buffer provided by Signosis, cell lysate can be prepared and high-through TF ELISA can be performed with limited number of cells from 96-well tissue plate.  Convenient and fast - Simple procedure with pre-coated plate included.

Nuclear Extraction Kit

Effective preparation of nuclear extracts, which is often needed for studying transcription factors.  Nuclear Extraction Kits can be used with our EMSA kits and plate assays.

Snapshot Microplate ChIP Assays

Effectively analyze the interaction of transcription factors on target promoters in a high-throughput plate format.

To simplify the ChIP assay in a high throughput method, Signosis has developed a 96-well microplate-based ChIP assay, where all of steps from IP to DNA elution are handled in microplate wells without sample transfers in an high throughput way. The assay is an ideal tool for studying up to 96 protein-DNA bindings in one sample or one protein-DNA binding in up to 96 samples, or multiple protein-DNA bindings in multiple samples/treatments.

Benefits:

• High Throughput - 96 ChIP experiments can be performed simultaneously in one microplate like ELISA.
• High Efficiency  - All steps from IP to DNA elution are done in a single well, including crosslinking and fragmentation without  transfering samples.
• High Reproducibility - Plate-based ChIP assay eliminates errors introduced from conventional multiple bead collection, wash and elution steps.
• Affordable - More economical per reaction than conventional ChIP assay.

Snapshot Magnetic Beads ChIP Assays

Analyze the interaction of transcription factors and associated coactivitors and corepressors on target promoters in 10⁶cells.

Benefits:

• High Reproducibility - Controllable MNase digestion for DNA fragmentation instead of sonication.
• High Specificity - The cross-linked protein-DNA complexes are subsequently pulled down by a specific antibody and ChIP grade protein A/G magnetic beads.
• Simple Procedure - Use one step Chelex-100 treatment to release cross-linked DNA instead of tedious proteinase K digestion and column purification.
• Convenience - Kit comes with everything you will need to perform the assay, including crosslinkers and enzyme.
• Dual cross linking to analyze both transcription factors and the associated factors.