Description(s):
These are human lung cancer cells derived from a 58 year old caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway.
Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.
Cell Line Origin
Human Caucasian lung carcinoma
Principle
| Cell line name | A549 |
| Catalog number | PC-003 |
| Species | Human |
| Tissue | Lung |
| Description | Tissue: Lung, Epithelial |
| Morphology/property | Epithelial-like/adherent |
| Growth | Continuous culture, grown as monolayer |
| Culture condition | Complete growth medium: In 450mL of DMEM, add 50mL FBS (10% final) and 5mL Penicillin/Streptomycin (1% final). |
| Subculturing | Subcultivation Ratio: 1:3 to 1:8 weekly |
| Medium Renewal: 2 to 3 times per week | |
| Hazard | Handle as Biosafety Level 1 containment |
| Storage Conditions | Liquid nitrogen |
| Freezing medium | Complete growth medium + 10% DMSO |
