Signosis

Product Name	Catalog #	Price		Qty
AP-1 Luciferase Reporter HeLa Stable Cell Line	SL-0019-FP	$2,904	X	- +

Description(s):

AP-1 Responsive Luciferase Reporter HeLa Stable Cell Line is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of AP-1 response element. This cell line is an ideal cellular model for monitoring the activation of JNK, ERK, MAPK Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Principle

AP-1 plays a key role in the control of cell proliferation, differentiation, transformation, survival and apoptosis. cJun and Fos dimerize to form AP-1 and this protein complex selectively binds to DNA sequences containing the TPA-responsive elements (TREs) to regulate gene expression. Many stimuli, both physiological and pathological, can regulate AP-1 activity, including TPA, serum, growth factors, cytokines, stress signals, infections, and oncoproteins, TNF and IL-1. AP-1 activity can be used as readout for JNK, ERK, or MAPK kinase signaling pathway.

Signosis has developed AP-1 luciferase reporter HeLa stable cell line by transducing cells with baculovirus containing both AP-1 luciferase reporter and hygromycin expression cassette. The hygromycin resistant clones were subsequently screened for PMA-induced luciferase activity. The cell line can be used as a reporter system for monitoring the activity of AP-1 triggered by stimuli treatment, such as TNFa, IL-1, gene overexpression and gene knockdown. The cells contain no viral particles and require handling at biosafety level 1 protocol.

Principle behind TF luciferase reporter. TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation.

Data

Analysis of AP-1 Luciferase Reporter HeLa Stable Cell Line. The cells were seeded on a 96-well plate for overnight with DMEM including 10% FBS. The cells then were treated with or without 10ng/ml PMA in DMEM and 0.1% FBS for 6 hours. More than 50 fold increase in luciferase activity was detected when compared to untreated cells. Data was read on a luminometer with a sensitivity of 3x10^-21 moles luciferase.

Literature

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Citations

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Targeted delivery of cisplatin to tumor xenografts via the nanoparticle component of nano-diamino-tetrac. Thangirala Sudha, Dhruba J Bharali, Murat Yalcin, Noureldien HE Darwish, Melis Debreli Coskun, Kelly A Keating, Hung-Yun Lin, Paul J Davis, Shaker A Mousa. February 2017 ,Vol. 12, No. 3, Pages 195-205 , DOI 10.2217/nnm-2016-0315.

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NFkB Luciferase Reporter A549 Stable Cell Lines (SL-0014)

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HEK293 TCF/LEF Luciferase Reporter Stable Cell Line (SL-0015)

Active β-catenin is regulated by the PTEN/PI3 kinase pathway: a role for protein phosphatase PP2A. Amit Persad,1,* Geetha Venkateswaran,1,* Li Hao,1 Maria E. Garcia,1 Jenny Yoon,1 Jaskiran Sidhu,1 and Sujata Persad1. Genes Cancer. 2016 Nov; 7(11-12): 368–382., doi: 10.18632/genesandcancer.128

Diallyl trisulfide inhibits proliferation, invasion and angiogenesis of glioma cells by inactivating Wnt/β-catenin signaling. Qingxia Tao, Cuiying Wu, Ruxiang Xu, Lijun Niu, Jiazhen Qin, Ning Liu, Peng Zhang, Chong Wang. Cell and Tissue Research, 2017, doi:10.1007/s00441-017-2678-9.

BCN057 induces intestinal stem cell repair and mitigates radiation-induced intestinal injury. Payel Bhanja, Andrew Norris, Pooja Gupta-Saraf, Andrew Hoover and Subhrajit Saha. Stem Cell Research & Therapy, vol. 9, no. 1, Feb. 2018, doi:10.1186/s13287-017-0763-3.

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Direct Antioxidant Properties of Methotrexate: Inhibition of Malondialdehyde-Acetaldehyde-Protein Adduct Formation and Superoxide Scavenging. Matthew C. Zimmermana, Dahn L. Clemensb, c, Michael J. Duryeeb, Cleofes Sarmientoa, Andrew Chioub, Carlos D. Hunterb, Jun Tiana, Lynell W. Klassenb, c, James R. O’Dellb, c, Geoffrey M. Thieleb, c, Redox Biology,2017.07.018, doi: 10.1016/j.redox.2017.07.018

Active β-catenin is regulated by the PTEN/PI3 kinase pathway: a role for protein phosphatase PP2A. Amit Persad1,*, Geetha Venkateswaran1,*, Li Hao1, Maria E. Garcia1, Jenny Yoon1, Jaskiran Sidhu1 and Sujata Persad1.Genes & Cancer. January 30, 2017 . https://doi.org/10.18632/genesandcancer.128

HIF Luciferase Reporter Hela Stable Cell Line (SL-0023)

Hypothermia augments stress response in mammalian cells. Eskla, Kattri-Liis, et al. Free Radical Biology and Medicine, 2018, doi:10.1016/j.freeradbiomed.2018.04.571.

NFkB Luciferase Reporter HepG2 Stable Cell Line (SL-0017)

Hypothermia augments stress response in mammalian cells. Eskla, Kattri-Liis, et al. Free Radical Biology and Medicine, 2018, doi:10.1016/j.freeradbiomed.2018.04.571.

CHOP Luciferase Reporter Mia_Paca2 Stable Cell Line (SL-0025)

A new ent-norabietant diterpenoid from roots of Euphorbia lathyris L. Yun, Young Sook, et al. Tetrahedron Letters, 2018, doi:10.1016/j.tetlet.2018.06.017.

NFAT Luciferase Reporter Jurkat Stable Cell Line (SL-0032)

Diversification of Bw4 Specificity and Recognition of a Nonclassical MHC Class I Molecule Implicated in Maternal–Fetal Tolerance by Killer Cell Ig-like Receptors of the Rhesus Macaque. Banerjee, Priyankana, et al. The Journal of Immunology, 2018, doi:10.4049/jimmunol.1800494.

P329G-CAR-J: a Novel Jurkat-NFAT-Based CAR-T Reporter System Recognizing the P329G Fc Mutation. Darowski, Diana, et al. Protein Engineering, Design and Selection, 2019, doi:10.1093/protein/gzz027.

Dendritic cells dictate responses to PD-L1 blockade cancer immunotherapy. Mayoux, Maud, et al. Science Translational Medicine Vol. 12, Issue 534, 2020.
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NRF2/ARE Luciferase Reporter HepG2 Stable Cell Line (SL-0046)

Innovative drinking water treatment techniques reduce the disinfection-induced oxidative stress and genotoxic activity. Lundqvist, Johan, et al. Water Research, vol. 155, 2019, pp. 182–192., doi:10.1016/j.watres.2019.02.052.

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Discovery of a New STAT3 Inhibitor Acting on the Linker Domain. Koseki, Tatsuya, et al. Biological and Pharmaceutical Bulletin, vol. 42, no. 5, 2019, pp. 792–800., doi:10.1248/bpb.b18-00992.

An engineered ligand trap inhibits leukemia inhibitory factor as pancreatic cancer treatment strategy. Hunter, S.A., McIntosh, B.J., Shi, Y. et al. Commun Biol 4, 452 (2021). https://doi.org/10.1038/s42003-021-01928-2

Stat1 Luciferase Reporter Hela Stable Cell Line (SL-0004)

Discovery of a New STAT3 Inhibitor Acting on the Linker Domain. Koseki, Tatsuya, et al. Biological and Pharmaceutical Bulletin, vol. 42, no. 5, 2019, pp. 792–800., doi:10.1248/bpb.b18-00992.

NRF2/ARE Luciferase Reporter NIH3T3 Stable Cell Line (SL-0047)

Thiamine and Benfotiamine Protect Neuroblastoma Cells against Paraquat and β-Amyloid Toxicity by a Coenzyme-Independent Mechanism. Sambon, Margaux, et al. Heliyon, vol. 5, no. 5, 2019, doi:10.1016/j.heliyon.2019.e01710.

NFkB Luciferase Reporter HEK293 Stable Cell Line (SL-0012)

Vitale LA, He LZ, Thomas LJ, et al. Development of CDX-527: a bispecific antibody combining PD-1 blockade and CD27 costimulation for cancer immunotherapy. Cancer Immunology, Immunotherapy : CII. 2020 May. DOI: 10.1007/s00262-020-02610-y.

Luciferase Substrate (LUC15/100)

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Targeting the Liver‐Brain Axis with Hop‐Derived Flavonoids Improves Lipid Metabolism and Cognitive Performance in Mice. Paraiso, I.L., Revel, J.S., Choi, J., Miranda, C.L., Lak, P., Kioussi, C., Bobe, G., Gombart, A.F., Raber, J., Maier, C.S. and Stevens, J.F. Mol. Nutr. Food Res. 2020, 2000341. https://doi.org/10.1002/mnfr.202000341

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Glass-bottled drinking water: a time capsule to study the historic presence of hazardous chemicals using effect-based methods. Lundqvist, J., Persson, K.M. & Oskarsson, A. Environ Sci Eur 33, 34 (2021). https://doi.org/10.1186/s12302-021-00476-0