Description(s):
AP-1, a dimeric protein complex composed of Jun and Fos proteins, plays a key role in the control of cell death, proliferation and transformation. Ap1 protein can be activated by many stimuli, including TPA, serum, growth factors and oncoproteins, TNF and IL-1, as well as by a variety of environmental stresses, such as UV radiation. When it is activated, AP1 protein binds to AP- DNA recognition element within the promoter regions of target genes and regulates the gene expression. Signosis has developed AP1 filter assay for monitoring DNA binding activity of AP1, which can be used for analyzing multiple samples simultaneously.
Principle
In Signosis' TF filter plate assays, a specific biotin-labeled TF DNA binding sequence is mixed with nuclear extracts to allow formation of TF-DNA complexes. A filter plate is used to retain bound DNA probe and remove free probe. The bound pre-labeled DNA probe is then eluted from the filter and collected for quantitative analysis through DNA plate hybridization. The captured DNA probe is further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer.
Data
Literature
View user manualCitations
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