Product Name Catalog # Price   Qty
Breast Cancer miRNA Plate Assay Kit I MA-0105 $623
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Characterization

Description(s):

It is estimated that about 30% of the mammalian gene expression is regulated by MicroRNAs, and aberrant expression of miRNAs has been observed in human cancers. Different types of cancers display different expression patterns of miRNAs.  According to the information from literature, Signosis developed a panel of miRNA plate assay kits; Each kit can be used to analyze 8 miRNA molecules related to a specific cancer type.


Applicable Grid:

 

Breast Cancer

Breast Cancer miRNA Plate Assay Kit I

miR-125a, miR-125b, miR-145, miR-146, miR-205, miR-206, miR-155, U6

Breast Cancer miRNA Plate Assay Kit II

miR-21, miR-10b, let7, miR-27b, miR-17-5p, miR-9-1, miR-126, U6

Breast Cancer miRNA Plate Assay Kit III

miR-200, miR-210, miR-335,  miR-373, miR-520c, U6

Lung Cancer

Lung Cancer miRNA Plate Assay I

let-7, miR-29, miR-34, miR-128b, miR-155, miR-200c, miR-221, U6

Lung Cancer miRNA Plate Assay II

miR-17-92 cluste

Colorectal Cancer

Colorectal Cancer miRNA Plate Assay I

miR-31, miR-96, miR-133b, miR-135b, miR-143, miR-145, miR-183, U6

Colorectal Cancer miRNA Plate Assay II

miR-17-92 cluster

Colorectal Cancer miRNA Plate Assay III

let-7a, let-7b, miR-15b, miR-21,  miR-26a, miR-30a, miR-30c, U6

Colorectal Cancer miRNA Plate Assay IV

miR-18, miR-27a, miR-34b, miR-106, miR-181b, miR-200c, miR-342, U6

Liver Cancer

Liver Cancer miRNA Plate Assay I

let-7c, miR-21, miR-23a, miR-24, miR-27a, miR-34a, miR-122a, U6

Liver Cancer miRNA Plate Assay II

miR-125a, miR-141, miR-199a, miR-200b, miR-221, miR-224, miR-320, U6

Pancreatic Cancer

Pancreatic Cancer miRNA Plate Assay I

miR-34a, miR-21, miR-155, miR-204, miR-221, miR-222, miR-301, U6

Pancreatic Cancer miRNA Plate Assay II

miR-155, miR-133a, miR-196a, miR-216, miR-217, miR-196b, miR-376a, U6

Bladder Cancer

Bladder Cancer miRNA Plate Assay I

miR-17; miR-23a, miR-23b; miR-26b; miR-103-1; miR-185; miR-203, U6

Ovarian Cancer

Ovarian Cancer miRNA Plate Assay I

let-7a-3, let-7b, miR-9, miR-18a, miR-93, miR-141, miR-199a, RNU48

Ovarian Cancer miRNA Plate Assay II

miR-100, miR-124a, miR-125b, miR-140, miR-141, miR-145, miR-199a, U6

Ovarian Cancer miRNA Plate Assay III

miR-200a, miR-200b, miR-200c,  miR-214, miR-302, miR-223, miR-429, RNU48

Prostate Cancer

Prostate Cancer miRNA Plate Assay I

let-7c, miR-34, miR-125b, miR-126, miR-145, miR-146a, miR-143, U6

Thyroid Cancer

 

Thyroid Cancer miRNA Plate Assay I

miR-21, miR-31, miR-221, miR-222, miR-181b, miR-221, miR-222, U6

Thyroid Cancer miRNA Plate Assay II

miR-30a, miR-30d; miR-125b; miR-26a, miR-197, miR-346, miR-138, U6

Lymphoma

 

Lymphoma miRNA Plate Assay I

let-7a, miR-16-1, miR-17, miR-21, miR-96, miR-106a, miR-155, U6

Lymphoma miRNA Plate Assay II

miR-17-92 clust

Leukemia

Leukemia miRNA Plate Assay I

let-7, miR-15, miR-16, miR-29, miR-142, miR-155, miR-181,U6

Leukemia miRNA Plate Assay II

miR-17-92 cluster

Leukemia miRNA Plate Assay III

miR-106-363 cluster

Leukemia miRNA Plate Assay IV

miR-107, miR-150, miR-181a, miR-181b, miR-128a, miR-128b, miR-223, U6

Leukemia miRNA Plate Assay V

miR-10a, miR-10b; miR-20a, miR-143, miR-145, miR-196a-1, miR-342, U6

 


Principle

In the proprietary miRNA plate assay, one miRNA molecule is flanked by a capture oligo and a biotinated detection oligo through two bridges oligos. One of the bridge oligos is partially hybridized with the miRNA molecule and the capture oligo and another with the miRNA and the detection oligo. The hybrid is immobilized onto plate through hybridization with an immobilized oligo and detected by a streptavidin-HRP conjugate and chemiluminecscent substrate. This hybrid structure is sensitive to the sequence of the miRNA molecule. One nucleotide difference will prevent the formation of the hybrid and therefore miRNA isoform can be differentiated, which normally is hard to tackle with Northern blot. In addition, the sensitivity of the assay is higher than miRNA Northern blot assay.