| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| Cancer Stem Cell TF Activation Profiling Plate Array | FA-1004 | $801 |
Characterization
Description(s):Transcriptional regulation plays a critical role in the control of biological processes, and transcription factors have been considered as master switches for cell fate determination. The cancer stem cell paradigm postulates that decontrolled tissue-specific stem cells or progenitor cells are precursors for cancer biogenesis. Cancer stem cells are a subset of cancer cells within a tumor that have stem cell-like characteristics. During tumorigenesis, deregulated transcription factor expression or activation can promote abnormal self-renewal, proliferation, and differentiation of the cells. For example, OCT4, Sox2,KLF4, Myc and Nanog have been shown to possess the power to reprogram somatic cells into pluripotent cells by reactivating pluripotent genes and silencing somatic genes. Understanding the activation patterns of cancer stem cell related transcription factors in a tumor can help to understand the transcriptional mechanism of cancer stem cell and provide better approaches for targeting and eliminating cancer stem cells.
Applicable Grid:
List of Applicable TFs:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | Androgen | KLF4 | Snail | Androgen | KLF4 | Snail | Androgen | KLF4 | Snail | Androgen | KLF4 | Snail |
| B | AP1 (Jun) | Myc | SOX2 | AP1 (Jun) | Myc | SOX2 | AP1 (Jun) | Myc | SOX2 | AP1 (Jun) | Myc | SOX2 |
| C | AP2 | Nanog | SOX9 | AP2 | Nanog | SOX9 | AP2 | Nanog | SOX9 | AP2 | Nanog | SOX9 |
| D | CREB | NFkB1 | STAT3 | CREB | NFkB1 | STAT3 | CREB | NFkB1 | STAT3 | CREB | NFkB1 | STAT3 |
| E | ER | NKX3.1 | TBX3 | ER | NKX3.1 | TBX3 | ER | NKX3.1 | TBX3 | ER | NKX3.1 | TBX3 |
| F | FoxO3 | Oct-3/4 | Twist-1 | FoxO3 | Oct-3/4 | Twist-1 | FoxO3 | Oct-3/4 | Twist-1 | FoxO3 | Oct-3/4 | Twist-1 |
| G | Gli | p53 | WT1 | Gli | p53 | WT1 | Gli | p53 | WT1 | Gli | p53 | WT1 |
| H | HIF-1 | PRDM14 | Blank | HIF-1 | PRDM14 | Blank | HIF-1 | PRDM14 | Blank | HIF-1 | PRDM14 | Blank |
Principle
Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.
Literature
View user manualCitations
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