Description(s):
Derived from CV-1 simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.
Cell Line Origin
Monkey African green kidney, SV40 transformed
Principle
| Cell line name | COS-7 | |
| Catalog number | PC-010 | |
| Species |
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| Tissue | Kidney | |
| Description | Tissue: kidney; Transformed by: SV-40 | |
| Morphology/property | Fibroblast/adherent | |
| Growth | Continuous culture, grown as monolayer | |
| Culture condition | Complete growth medium: In 450mL of DMEM, add 50mL FBS (10% final) and 5mL Penicillin/Streptomycin (1% final). | |
| Subculturing | Subcultivation Ratio: 1:4 to 1:8 weekly | |
| Medium Renewal: 2 to 3 times per week | ||
| Hazard | Handle as Biosafety Level 2 containment | |
| Storage Conditions | Liquid nitrogen | |
| Freezing medium | Complete growth medium + 10% DMSO |
