Description(s):

The plenti-EGFR (D770_N771insSVD, C797S) vector contains EGFR with the D770_N771insSVD, C797S mutations under the MSCV promoter which can be used to infect both dividing and nondividing cells. It contains the HIV-1 LTRs and the lentiviral packaging signal (Ψ) for retroviral expression, with specific elements (WPRE, cPPT, RRE) included for improved transgene expression. Downstream of the EGFR viii gene is a DYKDDDDK-tag for Western blotting. Furthermore, copGFP can be used as a selection marker when used with stable cell lines.

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death.  The EGFR D770_N771insSVD, C797S mutations involve the insertion of amino acids SVD (Ser, Val, Asp) between the position D770 and N771, and a point mutation that changes a C (Cys) into an S (Ser) at point 797. The C797S mutation in particular is known to confer resistance to EGFR inhibitors such as osimertinib and poziotinib.

 

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.