Description(s):

The pMSCV-EGFR (viii) vector contains the mutation EGFR viii under the MSCV promoter for high expression in hematopoietic, HEK293, and stem cells. Downstream of the EGFR viii gene is a DYKDDDDK-tag for Western blotting. Furthermore, the coGFP under the EF1 promoter can be used as a selection marker with stable cell lines. The base vector is a modified pMSCV vector with the LTRs and the lentiviral packaging signal (Ψ) removed for standard expression. For retroviral expression systems, please see our ELM series or contact customer support.

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death.  The EGFR viii mutation (also known as EGFR variant III, de2-7EGFR, and ΔEGFR) is one of the most common mutations found in human cancers. This mutation results in the overexpression of EGFR with an in-frame deletion of 801 base pairs representing exons 2-7. This deletion removes 267 amino acids and results in a significantly shorter extracellular domain that is unable to bind to any known ligand. 

 

 

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.