Description(s):

ELK Responsive Luciferase Reporter HEK293 Cell Line is derived human embryonic kidney, and stably express firefly luciferase reporter gene under the control of ELK response element. This cell line is an ideal cellular model for monitoring the activation of MAPK response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Principle

Mitogen-activated protein kinase (MAPK) signaling pathways mediate important eukaryotic transcriptional responses to extracellular signals that begin at the cell surface.  While different external stimuli may activate distinct MAPK signaling pathways, many MAPK pathways later converge in the activation of ETS domain transcription factor ELK1.  ELK1 is a major nuclear substrate for MAPKs, JNK, p38, and ERK pathways, where phosphorylation of ELK1 by these kinases results in a conformational change of ELK1 and triggers its DNA binding activity. 

Signosis has established an ELK-TAD luciferase reporter stable cell line.  This cell line expresses a fusion protein linking the DNA transactivation domain (TAD) of ELK to Gal4.  As the TAD is phosphorylated, this Gal4 fusion protein will drive luciferase expression controlled by Gal4-UAS.  This cell line can act as a reporter system to monitor ELK1-dependent transcriptional activity and MAPK signaling, as triggered by stimuli treatment, enforced gene expression and gene knockdown.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Data

 

 

 

 

 

 

 

 

 

 

Analysis of ELK-TAD Reporter Cell Line in response to MEKK overexpression. The cells were transfected with varying concentration of MEKK.  48 hours after transfection, cell lysate was collected to measure the luciferase activity.  ELK-TAD reporter activity showed a gene dose-dependent increase to MEKK overexpression. Data was read on a luminometer with a sensitivity of 3x10^-21 moles luciferase.

Literature

View user manual

 

 

Citations

NRF2/ARE Luciferase Reporter MCF7 Stable Cell Line (SL-0010)    Withaferin A induces Nrf2-dependent protection against liver injury: role of Keap1-independent mechanisms. DL Palliyaguru, DV Chartoumpekis, N Wakabayashi. Free Radical Biology and Medicine 2016. http://dx.doi.org/10.1016/j.freeradbiomed.2016.10.003   Occurrence and in vitro toxicity of unregulated disinfection by-products in two Saskatchewan drinking water treatment plants. Christena Leigh Watts. Handle Proxy, Universidade Da Coruña, 1 Sept. 2018, hdl.handle.net/10388/11499.   Luciferase Stable Expressing Hela Cell Line (SL-0102)   Redox-responsive, reversibly-crosslinked thiolated cationic helical polypeptides for efficient siRNA encapsulation and delivery.N Zheng, Z Song, Y Liu, R Zhang, R Zhang, C Yao. Journal of Controlled Release.Volume 205, 10 May 2015, Pages 231–239   Targeted delivery of cisplatin to tumor xenografts via the nanoparticle component of nano-diamino-tetrac. 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Genes Cancer. 2016 Nov; 7(11-12): 368–382., doi:  10.18632/genesandcancer.128   Diallyl trisulfide inhibits proliferation, invasion and angiogenesis of glioma cells by inactivating Wnt/β-catenin signaling. Qingxia Tao, Cuiying Wu, Ruxiang Xu, Lijun Niu, Jiazhen Qin, Ning Liu, Peng Zhang, Chong Wang. Cell and Tissue Research, 2017, doi:10.1007/s00441-017-2678-9.   BCN057 induces intestinal stem cell repair and mitigates radiation-induced intestinal injury. Payel Bhanja, Andrew Norris, Pooja Gupta-Saraf, Andrew Hoover and Subhrajit Saha. Stem Cell Research & Therapy, vol. 9, no. 1, Feb. 2018, doi:10.1186/s13287-017-0763-3.   (Nrf2/ARE) luciferase reporter cell line HEK293 (SL-0042)   Direct Antioxidant Properties of Methotrexate: Inhibition of Malondialdehyde-Acetaldehyde-Protein Adduct Formation and Superoxide Scavenging. Matthew C. Zimmermana, Dahn L. Clemensb, c, Michael J. Duryeeb, Cleofes Sarmientoa, Andrew Chioub, Carlos D. Hunterb, Jun Tiana, Lynell W. Klassenb, c, James R. O’Dellb, c, Geoffrey M. Thieleb, c, Redox Biology,2017.07.018, doi: 10.1016/j.redox.2017.07.018   Active β-catenin is regulated by the PTEN/PI3 kinase pathway: a role for protein phosphatase PP2A. Amit Persad1,*, Geetha Venkateswaran1,*, Li Hao1, Maria E. Garcia1, Jenny Yoon1, Jaskiran Sidhu1 and Sujata Persad1.Genes & Cancer. January 30, 2017 . https://doi.org/10.18632/genesandcancer.128    HIF Luciferase Reporter Hela Stable Cell Line (SL-0023)   Hypothermia augments stress response in mammalian cells. Eskla, Kattri-Liis, et al. Free Radical Biology and Medicine, 2018, doi:10.1016/j.freeradbiomed.2018.04.571.   NFkB Luciferase Reporter HepG2 Stable Cell Line (SL-0017)   Hypothermia augments stress response in mammalian cells. Eskla, Kattri-Liis, et al. Free Radical Biology and Medicine, 2018, doi:10.1016/j.freeradbiomed.2018.04.571.   CHOP Luciferase Reporter Mia_Paca2 Stable Cell Line (SL-0025)   A new ent-norabietant diterpenoid from roots of Euphorbia lathyris L. Yun, Young Sook, et al.  Tetrahedron Letters, 2018, doi:10.1016/j.tetlet.2018.06.017.   NFAT Luciferase Reporter Jurkat Stable Cell Line (SL-0032)   Diversification of Bw4 Specificity and Recognition of a Nonclassical MHC Class I Molecule Implicated in Maternal–Fetal Tolerance by Killer Cell Ig-like Receptors of the Rhesus Macaque. Banerjee, Priyankana, et al. The Journal of Immunology, 2018, doi:10.4049/jimmunol.1800494.   P329G-CAR-J: a Novel Jurkat-NFAT-Based CAR-T Reporter System Recognizing the P329G Fc Mutation. Darowski, Diana, et al. Protein Engineering, Design and Selection, 2019, doi:10.1093/protein/gzz027.   Dendritic cells dictate responses to PD-L1 blockade cancer immunotherapy. Mayoux, Maud, et al.  Science Translational Medicine Vol. 12, Issue 534, 2020. DOI: 10.1126/scitranslmed.aav7431    NRF2/ARE Luciferase Reporter HepG2 Stable Cell Line (SL-0046)   Innovative drinking water treatment techniques reduce the disinfection-induced oxidative stress and genotoxic activity. Lundqvist, Johan, et al. Water Research, vol. 155, 2019, pp. 182–192., doi:10.1016/j.watres.2019.02.052.   Stat3 Luciferase Reporter Hela Stable Cell Line (SL-0003)   Discovery of a New STAT3 Inhibitor Acting on the Linker Domain. Koseki, Tatsuya, et al. Biological and Pharmaceutical Bulletin, vol. 42, no. 5, 2019, pp. 792–800., doi:10.1248/bpb.b18-00992.   Stat1 Luciferase Reporter Hela Stable Cell Line (SL-0004)   Discovery of a New STAT3 Inhibitor Acting on the Linker Domain. Koseki, Tatsuya, et al. Biological and Pharmaceutical Bulletin, vol. 42, no. 5, 2019, pp. 792–800., doi:10.1248/bpb.b18-00992.   NRF2/ARE Luciferase Reporter NIH3T3 Stable Cell Line (SL-0047)   Thiamine and Benfotiamine Protect Neuroblastoma Cells against Paraquat and β-Amyloid Toxicity by a Coenzyme-Independent Mechanism. Sambon, Margaux, et al. Heliyon, vol. 5, no. 5, 2019, doi:10.1016/j.heliyon.2019.e01710.   NFkB Luciferase Reporter HEK293 Stable Cell Line (SL-0012)   Vitale LA, He LZ, Thomas LJ, et al. Development of CDX-527: a bispecific antibody combining PD-1 blockade and CD27 costimulation for cancer immunotherapy. Cancer Immunology, Immunotherapy : CII. 2020 May. DOI: 10.1007/s00262-020-02610-y.   Luciferase Substrate (LUC15/100)   Direct Antioxidant Properties of Methotrexate: Inhibition of Malondialdehyde-Acetaldehyde-Protein Adduct Formation and Superoxide Scavenging. Matthew C. Zimmermana, Dahn L. Clemensb, c, Michael J. Duryeeb, Cleofes Sarmientoa, Andrew Chioub, Carlos D. Hunterb, Jun Tiana, Lynell W. Klassenb, c, James R. O’Dellb, c, Geoffrey M. Thieleb, c, Redox Biology,2017.07.018, doi: 10.1016/j.redox.2017.07.018   FXR Luciferase Reporter HepG2 Stable Cell Line (SL-0055) Targeting the Liver‐Brain Axis with Hop‐Derived Flavonoids Improves Lipid Metabolism and Cognitive Performance in Mice. Paraiso, I.L., Revel, J.S., Choi, J., Miranda, C.L., Lak, P., Kioussi, C., Bobe, G., Gombart, A.F., Raber, J., Maier, C.S. and Stevens, J.F. Mol. Nutr. Food Res. 2020, 2000341. https://doi.org/10.1002/mnfr.202000341   Estrogen Receptor Luciferase Reporter T47D Stable Cell Line (SL-0002)   Glass-bottled drinking water: a time capsule to study the historic presence of hazardous chemicals using effect-based methods. Lundqvist, J., Persson, K.M. & Oskarsson, A. Environ Sci Eur 33, 34 (2021). https://doi.org/10.1186/s12302-021-00476-0