| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| ER Stress (UPR) TF Activation Profiling Plate Array | FA-1006 | $801 |
Characterization
Description(s):The Unfolded Protein Response (UPR) is a conserved and essential stress response that cells activate to combat endoplasmic reticulum (ER) stress, commonly caused by the accumulation of misfolded proteins or failing protein quality control. ER stress is a well-characterized feature of several diseases, such as diabetes, Alzheimer’s, Parkinson’s, Huntingon’s, and prion diseases. Early cellular response to ER stress includes the transcriptional upregulation of chaperone proteins, which is mediated by a large number of transcription factors (TFs).
Signosis has developed the Endoplasmic Reticulum Stress TF Activation Profiling Plate Array, which can be used to simultaneously monitor 16 ER stress/UPR related TFs, including XBP-1, ATF4, ATF6, GADD153/CHOP, CBF/NFY, SREBP1, YY1, ERR, ATF3, AP-1, FOXO1, IRF, p53, NFkB, NRF2/ARE, and HNF4.
Applicable Grid:
List of Applicable TFs:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | XBP-1 | ATF3 | XBP-1 | ATF3 | XBP-1 | ATF3 | XBP-1 | ATF3 | XBP-1 | ATF3 | XBP-1 | ATF3 |
| B | ATF4 | AP-1 | ATF4 | AP-1 | ATF4 | AP-1 | ATF4 | AP-1 | ATF4 | AP-1 | ATF4 | AP-1 |
| C | ATF6 | FOXO1 | ATF6 | FOXO1 | ATF6 | FOXO1 | ATF6 | FOXO1 | ATF6 | FOXO1 | ATF6 | FOXO1 |
| D | GADD153 | IRF | GADD153 | IRF | GADD153 | IRF | GADD153 | IRF | GADD153 | IRF | GADD153 | IRF |
| E | CBF/NFY | P53 | CBF/NFY | P53 | CBF/NFY | P53 | CBF/NFY | P53 | CBF/NFY | P53 | CBF/NFY | P53 |
| F | SREBP1 | NFkB | SREBP1 | NFkB | SREBP1 | NFkB | SREBP1 | NFkB | SREBP1 | NFkB | SREBP1 | NFkB |
| G | YY1 | NRF2/ARE | YY1 | NRF2/ARE | YY1 | NRF2/ARE | YY1 | NRF2/ARE | YY1 | NRF2/ARE | YY1 | NRF2/ARE |
| H | ERR | HNF4 | ERR | HNF4 | ERR | HNF4 | ERR | HNF4 | ERR | HNF4 | ERR | HNF4 |
Principle
Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.
Literature
View user manualCitations
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