| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| FewCell ER Stress (UPR) TF Activation Profiling Plate Array | FA-1106 | $802 |
Characterization
Description(s):The Unfolded Protein Response (UPR) is a conserved and essential stress response that cells activate to combat endoplasmic reticulum (ER) stress, commonly caused by the accumulation of misfolded proteins or failing protein quality control. ER stress is a well-characterized feature of several diseases, such as diabetes, Alzheimer’s, Parkinson’s, Huntingon’s, and prion diseases. Early cellular response to ER stress includes the transcriptional upregulation of chaperone proteins, which is mediated by a large number of transcription factors (TFs).
Signosis has developed the FewCell ER Stress/UPR TF Activation Profiling Plate Array, which can be used to simultaneously monitor 16 ER stress/UPR related TFs, including XBP-1, ATF4, ATF6, GADD153/CHOP, CBF/NFY, SREBP1, YY1, PGC-1a, ATF3, AP-1, FOXO1, IRF, p53, NFkB, NRF2/ARE, and HNF4. The array requires as few as 2,000 cells and the cellular protein is extracted with one-step procedure using the whole cell lysis buffer. The Kit contains one plate which can be used for 6 assays.
Applicable Grid:
List of Applicable TFs:
| 1 | 2 | 3 |
4 |
5 |
6 |
7 | 8 | 9 | 10 |
11 |
12 | |
| A | XBP-1 | ATF3 | XBP-1 |
ATF3 |
XBP-1 |
ATF3 |
XBP-1 | ATF3 | XBP-1 |
ATF3 |
XBP-1 |
ATF3 |
| B | ATF4 | AP-1 | ATF4 | AP-1 | ATF4 | AP-1 | ATF4 | AP-1 | ATF4 | AP-1 | ATF4 | AP-1 |
| C | ATF6 | FOXO1 |
ATF6 |
FOXO1 | ATF6 | FOXO1 | ATF6 | FOXO1 |
ATF6 |
FOXO1 | ATF6 | FOXO1 |
| D | GADD153 | MEF2 | GADD153 | MEF2 | GADD153 | MEF2 | GADD153 | MEF2 | GADD153 | MEF2 | GADD153 | MEF2 |
| E | CBF/NFY | p53 | CBF/NFY | p53 | CBF/NFY | p53 | CBF/NFY | p53 | CBF/NFY | p53 | CBF/NFY | p53 |
| F | SREBP1 | NFkB | SREBP1 | NFkB | SREBP1 | NFkB | SREBP1 | NFkB | SREBP1 | NFkB | SREBP1 | NFkB |
| G | YY1 | NRF2/ARE | YY1 | NRF2/ARE | YY1 | NRF2/ARE | YY1 | NRF2/ARE | YY1 | NRF2/ARE |
YY1 |
NRF2/ARE |
| H | ERR | HNF4 | ERR | HNF4 | ERR | HNF4 | ERR | HNF4 | ERR | HNF4 | ERR | HNF4 |
Principle
Signosis’ stem cell TF activation profiling plate array is used for monitoring the activation of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mix incubates with the cell lysate, individual probes will find its corresponding TF and form TF/probe complexes, which can be easily separated from free probes through simple spin column purification. The bound probes are detached from the complex and analyzed through hybridization with a plate; each well is specifically pre-coated with complementary sequences of the probes. The captured DNA probe is further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer.
