| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| FewCell Stem Cell TF Activation Profiling Plate Array | FA-1103 | $802 |
Characterization
Description(s):Stem cells are important cells characterized by their ability to self-renew or differentiate into many cell types, which is governed by intracellular signaling pathway and transcription regulation. Hence, transcription regulation plays a determining role in conferring cellular identity and function. The step-wise maturation of stem cells into terminally differentiated cell types requires the timely activation of a cascade of transcription programs governed by lineage-specifying transcription factors. Multiple transcription factors and target genes have been widely reported to associate with stem cell self-renewal and pluripotency including EGR1, OCT4, FOXD3, FOXO, Nanog, SOX2, SOX18, ETS, GLI, KLF4, MEF2, Myc, RNUX1, Pax6, TCF/LEF and GATA. However, stem cells are precious and limited.
Signosis has developed FewCell Stem Cell Transcription Factor Activation Plate Array to analyze activities of 16 stem cell-specific TFs simultaneously in mammalian samples. The array requires as few as 2,000 cells and the cellular protein is extracted with one-step procedure using the whole cell lysis buffer. This kit contains one plate which can be used for 6 assays.
Applicable Grid:
List of Applicable TFs:
| 1 | 2 | 3 |
4 |
5 |
6 |
7 | 8 | 9 | 10 |
11 |
12 | |
| A | EGR1 | Ets | EGR1 |
Ets |
EGR1 |
Ets |
EGR1 | Ets | EGR1 |
Ets |
EGR1 |
Ets |
| B | FOXD3 | Gli | FOXD3 | Gli | FOXD3 | Gli | FOXD3 | Gli | FOXD3 | Gli | FOXD3 | Gli |
| C | FOXO1 | KLF4 |
FOXO1 |
KLF4 | FOXO1 | KLF4 | FOXO1 | KLF4 |
FOXO1 |
KLF4 | FOXO1 | KLF4 |
| D | GATA | MEF2 | GATA | MEF2 | GATA | MEF2 | GATA | MEF2 | GATA | MEF2 | GATA | MEF2 |
| E | Nanog | Myc | Nanog | Myc | Nanog | Myc | Nanog | Myc | Nanog | Myc | Nanog | Myc |
| F | OCT4 | RNUX1 | OCT4 | RNUX1 | OCT4 | RNUX1 | OCT4 | RNUX1 | OCT4 | RNUX1 | OCT4 | RNUX1 |
| G | SOX2 | PAX6 | SOX2 | PAX6 | SOX2 | PAX6 | SOX2 | PAX6 | SOX2 | PAX6 |
SOX2 |
PAX6 |
| H | SOX18 | TCF/LEF | SOX18 | TCF/LEF | SOX18 | TCF/LEF | SOX18 | TCF/LEF | SOX18 | TCF/LEF | SOX18 | TCF/LEF |
Principle
Signosis’ stem cell TF activation profiling plate array is used for monitoring the activation of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mix incubates with the cell lysate, individual probes will find its corresponding TF and form TF/probe complexes, which can be easily separated from free probes through simple spin column purification. The bound probes are detached from the complex and analyzed through hybridization with a plate; each well is specifically pre-coated with complementary sequences of the probes. The captured DNA probe is further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer.
Data
Stem cell TF activation profiling plate array analysis of TF activities in HeLa and Stem cell F9. The whole cell lysates were prepared and subjected to TF profiling assay.
