Principle

Signosis has developed Firefly Luciferase Substrate for detecting firefly luciferase activity in our luciferase reporter product lines, including stable cell lines, TF reporter plasmids, and our Bac-In reporter systems, as well as other firefly reporters in the market.  This reagent produces a strong light signal that is comparable to competitors' substrates but at a more cost-effective price.

Data

 

HepG2 cells were transduced with Bac-In CMV-Luciferase for 24 hours and cells were lysed to assay for luciferase signal with the Signosis Firefly Luciferase Substrate.  Robust luciferase signal was detected after initial luminometer reading.  The signal showed little decay at 5 minutes post substrate addition and 80% of luciferase signal remained after 10 minutes.

Literature

View user manual

 

Citations

 

Direct Antioxidant Properties of Methotrexate: Inhibition of Malondialdehyde-Acetaldehyde-Protein Adduct Formation and Superoxide Scavenging. Matthew C. Zimmermana, Dahn L. Clemensb, c, Michael J. Duryeeb, Cleofes Sarmientoa, Andrew Chioub, Carlos D. Hunterb, Jun Tiana, Lynell W. Klassenb, c, James R. O’Dellb, c, Geoffrey M. Thieleb, c, Redox Biology,2017.07.018, doi: 10.1016/j.redox.2017.07.018