Description(s):
Human embryonic kidney cells 293, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy.
Cell Line Origin
Human Embryo Kidney
Principle
| Cell line name | HEK 293 |
| Catalog number | PC-002 |
| Species | Human |
| Tissue | Kidney |
| Description | Tissue: kidney, embryonic; Transformed by: adenovirus 5 |
| Morphology/property | Epithelial-like/adherent |
| Growth | Continuous culture, grown as monolayer |
| Culture condition | Complete growth medium: In 450mL of DMEM, add 50mL FBS (10% final) and 5 mL Penicillin/Streptomycin (1% final). |
| Subculturing | Subcultivation Ratio: 1:4 to 1:8 weekly Medium Renewal: Every 2 to 3 days |
| Hazard | Handle as Biosafety Level 2 containment |
| Storage Conditions | Liquid Nitrogen |
| Freezing medium | Complete Growth Medium + 10% DMSO |
