Description(s):
HeLa cells are human cancer cells derived from a cervical carcinoma from a 31 year old black female. This was the first aneuploid cell line derived from human tissue with ability to maintain in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. The cells should be handled under laboratory containment level 2.
Cell Line Origin
Human cervix epitheloid carcinoma
Principle
| Cell line name | HeLa |
| Catalog number | PC-001 |
| Species | human |
| Tissue | Cervix |
| Description | Tissue: Cervix, Epithelial; Transformed by: Human Papilloma virus 18 |
| Morphology/property | Epithelial-like/adherent |
| Growth | Continuous culture, grown as monolayer |
| Culture condition | Complete growth medium: In 450mL of DMEM, add 50mL FBS (10% final) and 5mL Penicillin/Streptomycin (1% final). |
| Subculturing | Subcultivation Ratio: 1:2 to 1:6 weekly |
| Medium Renewal: 2 to 3 times per week | |
| Hazard | Handle as Biosafety Level 2 containment |
| Storage Conditions | Liquid nitrogen |
| Freezing medium | Complete growth medium + 10% DMSO |
