Description(s):
The Hep G2 cell line was derived from the liver tissue of a 15-year-old Caucasian American male with a well-differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g. albumin, α2-macroglobulin, α 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. The cells will respond to stimulation with human growth hormone.
Cell Line Origin
Human Caucasian hepatocyte carcinoma
Principle
| Cell line name | HepG2 |
| Catalog number | PC-005 |
| Species | human |
| Tissue | Liver |
| Description | Tissue: Liver |
| Morphology/property | Epithelial-like/adherent |
| Growth | Continuous culture, grown as monolayer |
| Culture condition | Complete growth medium: In 450mL of EMEM (or DMEM), add 50mL FBS (10% final) and 5mL Penicillin/Streptomycin (1% final). |
| Subculturing | Subcultivation Ratio: 1:4 to 1:6 weekly |
| Medium Renewal: Twice per week | |
| Hazard | Handle as Biosafety Level 1 containment |
| Storage Conditions | Liquid nitrogen |
| Freezing medium | Complete growth medium + 10% DMSO |
