Description(s):
Hypoxia-inducible factor (HIF) has been recognized primarily for its role in the maintenance of oxygen and energy homoeostasis. HIF-1 and HIF-2 are heterodimeric transcription factors composed of HIF-1 and HIF-2 , respectively, which dimerize with a constitutively expressed ß subunit and subsequently bind to hypoxia response elements in the promoters of target genes. The activity of HIF is regulated by a variety of stimuli, including changes in cellular oxygen concentration, growth factors and oncogenic activation. Signosis has developed HIF filter assay. Unlike conventional gel shift assays, HIF filter assay is a high throughput assay and it can analyze DNA binding activities of HIF in multiple samples simultaneously. Meanwhile, the assay can be set up for a few samples with less tedious than non-radioactive gel shift assay or much safer than 32P based gel shift assay.
Principle
In Signosis' TF filter plate assays, a specific biotin-labeled TF DNA binding sequence is mixed with nuclear extracts to allow formation of TF-DNA complexes. A filter plate is used to retain bound DNA probe and remove free probe. The bound pre-labeled DNA probe is then eluted from the filter and collected for quantitative analysis through DNA plate hybridization. The captured DNA probe is further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer.
Data
Literature
View user manualCitations
1.Targeted Inhibition of Calpain Reduces Myocardial Hypertrophy and Fibrosis in Mouse Models of Type 1 Diabetes. Li Y, Ma J, Zhu H, Singh M, Hill D, Greer PA, Arnold JM, Abel ED, Peng T. Diabetes. 2011 Nov;60(11):2985-94.
2.Melatonin MT1 receptor-induced transcriptional up-regulation of p27Kip1 in prostate cancer antiproliferation is mediated via inhibition of constitutively active nuclear factor kappa B (NF-κB): potential implications on prostate cancer chemoprevention and therapy. Shiu SY, Leung WY, Tam CW, Liu VW, Yao KM. J Pineal Res. 2012 Jun 20. doi: 10.1111/j.1600-079X.2012.01026.x. [Epub ahead of print]
3.Deficiency of Capn4 Gene Inhibits Nuclear Factor-κB (NF-κB) Protein Signaling/Inflammation and Reduces Remodeling after Myocardial Infarction. Ma J, Wei M, Wang Q, Li J, Wang H, Liu W, Lacefield JC, Greer PA, Karmazyn M, Fan GC, Peng T. J Biol Chem. 2012 Aug 10;287(33):27480-9.
4.Anti-inflammatory salicylate treatment alters the metabolic adaptations to lactation in dairy cattleAmerican Journal of Physiology - Regulatory, Integrative and Comparative Physiology 2013.July 305(2) R110
5.Involvement of a chromatin modifier in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell injury: Probably an indirect action via the regulation of NFκB/FasL circuitry. Chen S, Dong Y, Xu C, Jiang L, Chen Y, Jiang C, Hou W, Li W. Biochem Biophys Res Commun. 2013 Oct 8. pii: S0006-291X(13)01640-9.
6.Immunoglobulin light chains activate nuclear factor-B in renal epithelial cells through a Src-dependent mechanism. Ying WZ, Wang PX, Aaron KJ, Basnayake K, Sanders PW. Blood. 2011 Jan 27;117(4):1301-7.
7. In vivo selective imaging and inhibition of leukemia stem-like cells using the fluorescent carbocyanine derivative, DiOC5 (3). B Zhang, Y Shimada, J Kuroyanagi, M Ariyoshi . Biomaterials, 2015. Volume 52, June 2015, Pages 14–25
8. Repression of a chromatin modifier aggravates lipopolysaccharide-induced acute lung injury in mouseJia-kuan Chena, 1, Wen-chen Wanga, 1, Li Zangb, Jie Zhaoc, Wei Lic, Tao Jianga, ,Biochemical and Biophysical , 2016. doi:10.1016/j.bbrc.2016.02.043
9. Repression of a chromatin modifier aggravates lipopolysaccharide-induced acute lung injury in mouse
J Chen, W Wang, L Zang, J Zhao, W Li, T Jiang . Biochemical and Biophysical Research Communications 2016. doi:10.1016/j.bbrc.2016.02.043
10. Bmi1 inhibition enhances the sensitivity of pancreatic cancer cells to gemcitabine
Tao Yin, Zhengle Zhang, Bin Cao, Qingke Duan, Pengfei Shi, Hengqiang Zhao, Soriba Naby Camara, Qiang Shen, Chunyou Wang
Oncotarget. 2016 May 11. doi: 10.18632/oncotarget.9293
11. Protective effects of tyrosol against LPS-induced acute lung injury via inhibiting NF-κB and AP-1 activation and activating the HO-1/Nrf2 Pathways. Wen-chen Wanga, Yan-min Xiaa , Bo Yangb, Xiang-ni Suc , Jia-kuan Chena, Wei Li, Tao Jiang. Biological & Pharmaceutical Bulletin Biological and Pharmaceutical Bulletin 2017 DOI:10.1248/bpb.b16-00756
12. Adipose Tissue-specific Inhibition of Hypoxia-inducible Factor 1 Induces Obesity and Glucose Intolerance by Impeding Energy Expenditure in Mice. Zhang X, Lam KS, Ye H, Chung SK, Zhou M, Wang Y, Xu A. J Biol Chem. 2010 Oct 22;285(43):32869-77.
13. HIF-2α Suppresses p53 to Enhance the Stemness and Regenerative Potential of Human Embryonic Stem Cells. Das B, Bayat-Mokhtari R, Tsui M, Lotfi S, Tsuchida R, Felsher DW, Yeger H. Stem Cells. 2012 Aug;30(8):1685-1695.
14. Protective effects of tyrosol against LPS-induced acute lung injury via inhibiting NF-κB and AP-1 activation and activating the HO-1/Nrf2 Pathways. Wen-chen Wanga, Yan-min Xiaa , Bo Yangb, Xiang-ni Suc , Jia-kuan Chena, Wei Li, Tao Jiang. Biological & Pharmaceutical Bulletin Biological and Pharmaceutical Bulletin 2017 DOI:10.1248/bpb.b16-00756
15. Upregulation of miRNA-155 expression by OxLDL in dendritic cells involves JAK1/2 kinase and transcription factors YY1 and MYB. H Yan, S Wang, Z Li, W Zhao, Z Wang, Z Sun, Y Pan. International Journal of Molecular Medicine , Print ISSN: 1107-3756. Online ISSN:1791-244X, Published online on: Friday, March 11, 2016
16. Sa1684 Common Food Additive Carrageenan Stimulates Wnt/β-Catenin Signaling Pathway in Colonic Epithelium by Inhibition of Nucleoredoxin Reduction.L Feferman, JK Tobacman. Nutrition and Cancer, 1–11, 2014, ISSN: 0163-5581 print / 1532-7914 online. DOI: 10.1080/01635581.2014.852228
17. CRP promotes MMP-10 expression via c-Raf/MEK/ERK and JAK1/ERK pathways in cardiomyocytes.Cui C, Shi Q, Zhang X, Liu X, Bai Y, Li J, Liu S, Hu S, Wei Y. Cell Signal. 2012 Mar;24(3):810-8.
18. Chondroitin sulfatases differentially regulate Wnt signaling in prostate stem cells through effects on SHP2, phospho-ERK1/2, and Dickkopf Wnt signaling pathway inhibitor (DKK3). Bhattacharyya, Sumit, et al. Oncotarget, 2017, doi:10.18632/oncotarget.22152.
