How to order this kit:
Step 1: choose miRNA Northern Blot
| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| Highly sensitive miRNA Northern Blot Assay Kit without gels | NB-1002 | $486 |
Step 2: choose oligo for miRNA of interest
| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| miR-153-3p(HS) | HP-0077 | $204 | ||
| miR-152-3p(HS) | HP-0078 | $204 | ||
| miR-154-5p(HS) | HP-0079 | $204 | ||
| miR-155-5p(HS) | HP-0080 | $204 | ||
| miR-181a-5p(HS) | HP-0081 | $204 | ||
| miR-181b-5p(HS) | HP-0082 | $204 | ||
| miR-181c-5p(HS) | HP-0083 | $204 | ||
| miR-181d-5p(HS) | HP-0084 | $204 | ||
| miR-182-5p(HS) | HP-0085 | $204 | ||
| miR-183-5p(HS) | HP-0086 | $204 | ||
| miR-184(HS) | HP-0087 | $204 | ||
| miR-185-5p(HS) | HP-0088 | $204 | ||
| miR-186-5p(HS) | HP-0089 | $204 | ||
| miR-188-5p(HS) | HP-0090 | $204 | ||
| miR-190-5p(HS) | HP-0091 | $204 | ||
| miR-191-5p(HS) | HP-0092 | $204 | ||
| miR-192-5p(HS) | HP-0093 | $204 | ||
| miR-193a-3p(HS) | HP-0094 | $204 | ||
| miR-193a-5p(HS) | HP-0095 | $204 | ||
| miR-194-5p(HS) | HP-0096 | $204 | ||
| miR-195-5p(HS) | HP-0097 | $204 | ||
| miR-196a-5p(HS) | HP-0098 | $204 | ||
| miR-196b-5p(HS) | HP-0099 | $204 | ||
| miR-197-3p(HS) | HP-0100 | $204 | ||
| miR-198(HS) | HP-0101 | $204 | ||
| miR-199a*-3p(HS) | HP-0102 | $204 | ||
| miR-199a-5p(HS) | HP-0103 | $204 | ||
| miR-199b-5p(HS) | HP-0104 | $204 | ||
| miR-200a-3p(HS) | HP-0105 | $204 | ||
| miR-200c-3p(HS) | HP-0106 | $204 | ||
| miR-202-5p(HS) | HP-0107 | $204 | ||
| miR-203a(HS) | HP-0108 | $204 | ||
| miR-204-5p(HS) | HP-0109 | $204 | ||
| miR-205-5p(HS) | HP-0110 | $204 | ||
| miR-206(HS) | HP-0111 | $204 | ||
| miR-210-3p(HS) | HP-0112 | $204 | ||
| miR-213 (miR-181a-3p)(HS) | HP-0113 | $204 | ||
| miR-92-3p(HS) | HP-0036 | $185 | ||
| miR-214-3p(HS) | HP-0114 | $204 | ||
| miR-215-5p(HS) | HP-0115 | $204 | ||
| miR-216-5p(HS) | HP-0116 | $204 | ||
| miR-218-5p(HS) | HP-0117 | $204 | ||
| miR-92b-3p(HS) | HP-0037 | $185 | ||
| miR-219(HS) | HP-0118 | $204 | ||
| miR-221-3p(HS) | HP-0119 | $204 | ||
| miR-222-3p(HS) | HP-0120 | $204 | ||
| miR-223-3p(HS) | HP-0121 | $204 | ||
| miR-93-5p(HS) | HP-0038 | $185 | ||
| miR-224-5p(HS) | HP-0122 | $204 | ||
| miR-296-5p(HS) | HP-0123 | $204 | ||
| miR-329(HS) | HP-0124 | $204 | ||
| miR-337-3p(HS) | HP-0125 | $204 | ||
| miR-95-3p(HS) | HP-0039 | $185 | ||
| miR-338(HS) | HP-0126 | $204 | ||
| miR-342-3p(HS) | HP-0127 | $204 | ||
| miR-345-5p(HS) | HP-0128 | $204 | ||
| miR-376c-3p (miR-368) (HS) | HP-0129 | $204 | ||
| miR-96-5p(HS) | HP-0040 | $185 | ||
| miR-369-3p(HS) | HP-0130 | $204 | ||
| miR-373-5p(HS) | HP-0134 | $204 | ||
| miR-375(HS) | HP-0135 | $204 | ||
| miR-488-5p(HS) | HP-0136 | $204 | ||
| miR-99a-5p(HS) | HP-0041 | $185 | ||
| miR-497-5p(HS) | HP-0137 | $204 | ||
| miR-499-5p(HS) | HP-0138 | $204 | ||
| miR-507(HS) | HP-0139 | $204 | ||
| miR-609(HS) | HP-0140 | $204 | ||
| miR-100-5p(HS) | HP-0042 | $185 | ||
| miR-720(HS) | HP-0141 | $204 | ||
| miR-760(HS) | HP-0142 | $204 | ||
| miR-27b-3p(HS) | HP-0143 | $204 | ||
| miR-4767 (HS) | HP-0144 | $204 | ||
| miR-101-3p(HS) | HP-0043 | $185 | ||
| U6(HS) | HP-1001 | $204 | ||
| sno234(HS) | HP-1002 | $204 | ||
| RNU48(HS) | HP-1003 | $204 | ||
| miR-103-3p(HS) | HP-0044 | $185 | ||
| miR-106a-5p(HS) | HP-0045 | $185 | ||
| miR-106b-5p(HS) | HP-0046 | $185 | ||
| miR-107(HS) | HP-0047 | $185 | ||
| miR-122a-5p(HS) | HP-0048 | $185 | ||
| miR-124a-3p(HS) | HP-0049 | $185 | ||
| miR-125a-5p(HS) | HP-0050 | $185 | ||
| let-7a-5p(HS) | HP-0001 | $185 | ||
| miR-125b-5p(HS) | HP-0051 | $185 | ||
| let-7b-5p(HS) | HP-0002 | $185 | ||
| miR-126-3p(HS) | HP-0052 | $185 | ||
| let-7c-5p(HS) | HP-0003 | $185 | ||
| let-7d-5p(HS) | HP-0004 | $185 | ||
| miR-127-5p(HS) | HP-0053 | $185 | ||
| let-7e-5p(HS) | HP-0005 | $185 | ||
| miR-128a-3p(HS) | HP-0054 | $185 | ||
| let-7f-5p(HS) | HP-0006 | $185 | ||
| miR-128b-3p(HS) | HP-0055 | $185 | ||
| let-7g-5p(HS) | HP-0007 | $185 | ||
| let-7i-5p(HS) | HP-0008 | $185 | ||
| miR-131(HS) | HP-0056 | $185 | ||
| miR-1 (HS) | HP-0009 | $185 | ||
| miR-132-3p(HS) | HP-0057 | $185 | ||
| miR-7-5p(HS) | HP-0010 | $185 | ||
| miR-9-5p(HS) | HP-0011 | $185 | ||
| miR-133a-3p(HS) | HP-0058 | $185 | ||
| miR-10a-5p(HS) | HP-0012 | $185 | ||
| miR-133b(HS) | HP-0059 | $185 | ||
| miR-15a-5p(HS) | HP-0013 | $185 | ||
| miR-134-5p(HS) | HP-0060 | $185 | ||
| miR-16-5p(HS) | HP-0014 | $185 | ||
| miR-17-3p(HS) | HP-0015 | $185 | ||
| miR-135b-5p(HS) | HP-0061 | $185 | ||
| miR-17-5p(HS) | HP-0016 | $185 | ||
| miR-136-5p(HS) | HP-0062 | $185 | ||
| miR-18a-5p(HS) | HP-0017 | $185 | ||
| miR-137(HS) | HP-0063 | $185 | ||
| miR-19a-3p(HS) | HP-0018 | $185 | ||
| miR-20a-5p(HS) | HP-0019 | $185 | ||
| miR-140-5p(HS) | HP-0064 | $185 | ||
| miR-21-5p(HS) | HP-0020 | $185 | ||
| miR-141-3p(HS) | HP-0065 | $185 | ||
| miR-22-3p(HS) | HP-0021 | $185 | ||
| miR-142-3p(HS) | HP-0066 | $185 | ||
| miR-23a-3p(HS) | HP-0022 | $185 | ||
| miR-24-3p(HS) | HP-0023 | $185 | ||
| miR-142-5p(HS) | HP-0067 | $185 | ||
| miR-25-3p(HS) | HP-0024 | $185 | ||
| miR-143-3p(HS) | HP-0068 | $185 | ||
| miR-26a-5p(HS) | HP-0025 | $185 | ||
| miR-144-3p(HS) | HP-0069 | $185 | ||
| miR-27a-3p(HS) | HP-0026 | $185 | ||
| miR-28-5p(HS) | HP-0027 | $185 | ||
| miR-145-5p(HS) | HP-0070 | $185 | ||
| miR-29a-3p(HS) | HP-0028 | $185 | ||
| miR-146a-5p(HS) | HP-0071 | $185 | ||
| miR-29b-3p(HS) | HP-0029 | $185 | ||
| miR-29c-3p(HS) | HP-0030 | $185 | ||
| miR-30a-3p(HS) | HP-0031 | $185 | ||
| miR-148a-3p(HS) | HP-0073 | $185 | ||
| miR-30a-5p(HS) | HP-0032 | $185 | ||
| miR-149-5p(HS) | HP-0074 | $185 | ||
| miR-30b-5p(HS) | HP-0033 | $185 | ||
| miR-150-5p(HS) | HP-0075 | $185 | ||
| miR-30c-5p(HS) | HP-0034 | $185 | ||
| miR-31-5p(HS) | HP-0035 | $185 | ||
| miR-151-3p(HS) | HP-0076 | $185 |
Abundance of mammalian miRNAs varies dramatically in tissues. For example, expression of miR-133 is high in skeletal muscle, moderate in heart, and no detection in other tissues. To detect low expression of miRNAs requires high sensitive methods. Signosis’ highly sensitive miRNA northern blot assay is 10 to 100 times more sensitive than conventional biotin-based chemiluminescent detection.
To do the assay, you need the miRNA Northern Blot Assay Kit and probes for specific miRNAs.
Note: Our miRNA Northern Blot kits no longer come with precast gels. It is highly recommended for our customers to provide their own gels instead. Freshly made gels can be altered and refined to better suit our customer's experiments. Instructions on creating your own gel are also included in the manual which can be used as a guideline. If kits with precast gels are still desired, please contact us for pricing and availability.
Principle
RNA samples are separated through gel electrophoresis and transferred onto a membrane. Expression of a specific miRNA is detected with a biotin-labeled probe. This regular Northern blot assay is suitable to most moderate to high expressors of miRNA. High sensitive Northern blot (NB-1001 and HP-XXXX) is needed to analyze low expressors of miRNA. The biotin-labeled probes contain two moieties – complementary sequence of the miRNA and a tag sequence. The tag sequence is then detected by an amplifier enriched with biotin molecules.
Data
Highly sensitive miRNA northern blot analysis of miR-133 in skeletal muscle total RNA and miR-21 from Hela cell total RNA.
Literature
View user manualCitations
Identification and Characterization of High Temperature Stress Responsive Novel MiRNAs in French Bean (Phaseolus Vulgaris). Jyothi, M. N., D. V. Rai, and R. Nagesh Babu. Appl Biochem Biotechnol Applied Biochemistry and Biotechnology 176.3 (2015): 835-49.;
miR-1916, miR-3217, miR-1164, miR-2844
Global Identification of Ginkgo Biloba MicroRNAs and Insight into Their Role in Metabolism Regulatory Network of Terpene Trilactones by High-throughput Sequencing and Degradome Analysis. Ye, Jiabao, Xian Zhang, Junping Tan, Feng Xu, Shuiyuan Cheng, Zexiong Chen, Weiwei Zhang, and Yongling Liao. Industrial Crops and Products 148 (2020): 112289.
miR-21 and miR-181b
NFI-A disrupts myeloid cell differentiation and maturation in septic mice.C McClure, E Ali, D Youssef, ZQ Yao, CE McCall. Journal of Leukocyte Biology.Published online before print August 10, 2015, doi: 10.1189/jlb.4A0415-171RR
jlb.4A0415-171RR
miR-23b
MicroRNA-23b mediates urokinase and c-met downmodulation and a decreased migration of human hepatocellular carcinoma cells Alessandro Salvi, Cristiano Sabelli, Silvia Moncini, Marco Venturin, Bruna Arici, Paola Riva, Nazario Portolani, Stefano M. Giulini, Giuseppina De Petro, Sergio Barlati First published: 16 April 2009 DOI: 10.1111/j.1742-4658.2009.07014.x
miR-34a
A metabolic stress-inducible miR-34a-HNF4α pathway regulates lipid and lipoprotein metabolism. Yang Xu, Munaf Zalzala, Jiesi Xu, Yuanyuan Li, Liya Yin & Yanqiao Zhang , Nature Communications 6,Article number:7466
doi:10.1038/ncomms8466 Published 23 June 2015
miR-26a , miR-30a, miR-34a
Stress Induces p38 MAPK-Mediated Phosphorylation and Inhibition of Drosha-Dependent Cell Survival
Q Yang, W Li, H She, J Dou, DM Duong, Y Du… - Molecular Cell, 2015
miRNA-233
Identification and comparative analysis of differentially expressed miRNAs in leaves of two wheat (Triticum aestivum L.) genotypes during dehydration stress.X Ma, Z Xin, Z Wang, Q Yang, S Guo, X Guo, L Cao… - BMC Plant Biology, 2015
miR-940
MicroRNA-940 suppresses prostate cancer migration and invasion by regulating MIEN1. S Rajendiran, AV Parwani, RJ Hare, S Dasgupta… - Molecular Cancer, 2014
miR-134
The miR-134 attenuates the expression of transcription factor FOXM1 during pluripotent NT2/D1 embryonal carcinoma cell differentiation. Y Chen, L Meng, Q Yu, D Dong, G Tan, X Huang, Y Tan - Experimental Cell Research, 2014
miR-27b
Gingerolinduced myeloid leukemia cell death is initiated by reactive oxygen species and activation of miR-27b expression. Rastogi N, Gara RK, Trivedi R, Singh A, Dixit P, Maurya R, Duggal S, Bhatt ML, Singh S, Mishra DP. Free Radic Biol Med. 2014 Mar;68:288-301.
miR-155
Deletion of microRNA-155 reduces autoantibody responses and alleviates lupus-like disease in the Faslpr mouse. Thai TH, Patterson HC, Pham DH, Kis-Toth K, Kaminski DA, Tsokos GC. Proc Natl Acad Sci U S A. 2013 41983;110(50):20194-9.
miR-142-3p
Selective Depletion of CD4+CD25+Foxp3+ Regulatory T Cells by Low-Dose Cyclophosphamide Is Explained by Reduced Intracellular ATP Levels. Zhao J, Cao Y, Lei Z, Yang Z, Zhang B, Huang B. Cancer Res. 2010 Jun 15;70(12):4850-8.
miRNA-223 and sno234
Impaired Micro-RNA Pathways Diminish Osteoclast Differentiation and Function. Sugatani T, Hruska KA. J Biol Chem. 2009 Feb 13;284(7):4667-78.
let-7e
miRNA let-7e Modulates the Wnt Pathway and Early Nephrogenic Markers in Mouse Embryonic Stem Cell Differentiation. Viñas JL, Ventayol M, Brüne B, Jung M, Sola A, Pi F, Mastora C, Hotter G.. PLoS One. 2013 Apr-10;8(4): e60937
miR159, miR167, miR169, miR172
Expression of miRNAs regulates Growth and Development of French bean (Phaseolus vulgaris) under Salt and Drought Stress conditions. Nageshbabu R, Jyothi MN, Sharadamma N, Sahu Sarika, Rai DV, Devaraj VR. Int. Res. J. Biological Sci. 2013 Jan;2(1): 52-56.
mir-17-92
Efficient inhibition of HIV-1 replication by an artificial polycistronic miRNA construct. Zhang T, Cheng T, Wei L, Cai Y, Yeo AE, Han J, Yuan YA, Zhang J, Xia N. Virol J. 2012 Jun 18;9:118.
miR-29b
OxLDL causes both epigenetic modification and signaling regulation on the microRNA-29b gene: Novel mechanisms for cardiovascular diseases. Chen KC, Liao YC, Hsieh IC, Wang YS, Hu CY, Juo SH. J Mol Cell Cardiol. 2012 Mar;52(3):587-95.
miR-145
1.Glucocorticoid regulation of a novel HPV–E6–p53–miR-145 pathway modulates invasion and therapy resistance of cervical cancer cells. Shi M, Du L, Liu D, Qian L, Hu M, Yu M, Yang Z, Zhao M, Chen C, Guo L, Wang L, Song L, Ma Y, Guo N. J Pathol. 2012 Oct;228(2):148-57. doi: 10.1002/path.3997. Epub 2012 Apr 18.
2.Expression of Micro-RNA-145 is regulated by a highly conserved genomic sequence 3′ to the pre-miR . La Rocca G, Shi B, Sepp-Lorenzino L, Baserga R. J Cell Physiol. 2011 Mar;226(3):602-7.
miR-181
Functional analysis of microRNAs in human hepatocellular cancer stem cells. Meng F, Glaser SS, Francis H, DeMorrow S, Han Y, Passarini JD, Stokes A, Cleary JP, Liu X, Venter J, Kumar P, Priester S, Hubble L, Staloch D, Sharma J, Liu CG, Alpini G. J Cell Mol Med. 2012 Jan;16(1):160-73.
let-7g
Negative feedback regulation between microRNA let-7g and the oxLDL receptor LOX-1. Chen KC, Hsieh IC, Hsi E, Wang YS, Dai CY, Chou WW, Juo SH. J Cell Sci. 2011 Dec 1;124(Pt 23):4115-24.
miR166b, miR169d, miR482b, miR1507a
Characterization of the stress associated microRNAs in Glycine max by deep sequencing. Li H, Dong Y, Yin H, Wang N, Yang J, Liu X, Wang Y, Wu J, Li X. BMC Plant Biol. 2011 Nov 23;11:170
let-7a
Latency-associated nuclear antigen inhibits lytic replication of Kaposi's sarcoma-associated herpesvirus by regulating let-7a/RBPJ signaling. Qi, Yan, et al. Virology, 2019, doi:10.1016/j.virol.2019.02.019.
miRNA Northern Blot Assay Kit NB-1001 and NB-1002
Retrograde signaling by a mtDNA-encoded non-coding RNA preserves mitochondrial bioenergetics. Blumental-Perry, A., Jobava, R., Bederman, I. et al. Commun Biol 3, 626 (2020). https://doi.org/10.1038/s42003-020-01322-4
