| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| Human Angiogenesis ELISA Plate Array (Colorimetric) | EA-4012 | $946 |
Characterization
Description(s):Angiogenesis provides the crucial link between the avascular and vascular states and is a key event for sustained tumor growth and cancer progression. Angiogenesis as a biological switch process is governed by numerous pro- and anti-angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGFb), epidermal growth factor (EGF), and transforming growth factor-beta (TGF-b). The mechanism of action of each of these factors is different, as are their origin and the stimuli for their production. The angiogenic switch refers to the balance between pro- and anti- angiogenic factors. Therefore, profiling of these factors is critical to understanding angiogenesis. Signosis’ Angiogenesis ELISA Plate Array allows simultaneously profiling 16 angiogenesis cytokines. Each well is coated with a primary antibody against a specific angiogenesis protein and total 16 wells of a target 16 different proteins. The difference of these proteins between two samples can be determined through data comparison.
Detection Range:
8 - 2000 pg/mL
Sensitivity:
5 - 10 pg/mL
Applicable Grid:
List of Applicable Cytokines
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | TNFa | PDGF-BB | TNFa | PDGF-BB | TNFa | PDGF-BB | TNFa | PDGF-BB | TNFa | PDGF-BB | TNFa | PDGF-BB |
| B | IGF-1 | PIGF-1 | IGF-1 | PIGF-1 | IGF-1 | PIGF-1 | IGF-1 | PIGF-1 | IGF-1 | PIGF-1 | IGF-1 | PIGF-1 |
| C | VEGF | b-NGF | VEGF | b-NGF | VEGF | b-NGF | VEGF | b-NGF | VEGF | b-NGF | VEGF | b-NGF |
| D | IL-6 | SCF | IL-6 | SCF | IL-6 | SCF | IL-6 | SCF | IL-6 | SCF | IL-6 | SCF |
| E | FGFb | MCP-1 | FGFb | MCP-1 | FGFb | MCP-1 | FGFb | MCP-1 | FGFb | MCP-1 | FGFb | MCP-1 |
| F | TGFb | MIP-1a | TGFb | MIP-1a | TGFb | MIP-1a | TGFb | MIP-1a | TGFb | MIP-1a | TGFb | MIP-1a |
| G | EGF | IL-2 | EGF | IL-2 | EGF | IL-2 | EGF | IL-2 | EGF | IL-2 | EGF | IL-2 |
| H | Leptin | IL-4 | Leptin | IL-4 | Leptin | IL-4 | Leptin | IL-4 | Leptin | IL-4 | Leptin | IL-4 |
Principle
The 96-well clear plate is divided into 6 sections, and each section has 2 columns for one sample. In each section, 16 specific angiogenesis cytokine capture antibodies are coated on 16 wells respectively. The sample, cell culture supernatants, cell lysates, tissue homogenates, serum, or plasma samples among others are incubated with the cytokine ELISA plate, and the captured cytokine proteins are subsequently detected with a cocktail of biotinylated detection antibodies. The test sample is allowed to react with a pair of antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The plate is further detected with HRP luminescent substrate or HRP substrate TMB. The level of expression for each specific cytokine is directly proportional to the luminescent or color intensity.
Literature
View user manualCitation:
Mesenchymal Stem Cell-derived Magnetic Extracellular Nanovesicles for Targeting and Treatment of Ischemic Stroke. Kim, Han Young, Tae Jung Kim, Lami Kang, Young-Ju Kim, Min Kyoung Kang, Jonghoon Kim, Ju Hee Ryu, Taeghwan Hyeon, Byung-Woo Yoon, Sang-Bae Ko, and Byung-Soo Kim. Biomaterials (2020): 119942.
