| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| Human Cancer Cytokine ELISA Plate Array (Colorimetric) | EA-4016 | $946 |
Characterization
Description(s):Cytokines produced in the cancer microenvironment play a crucial role in cancer pathogenesis. Cancer cells can lead to abnormal cytokine production that promote growth, attenuate apoptosis and facilitate invasion and metastasis. The clinic studies have revealed that cancer patients often experience a simultaneous immunostimulation and immunosuppression with different cytokine expression patterns. The various patterns are closely associated with cancer origin and pathological grades and stages. Signosis developed Cancer Cytokine ELISA Array, which can quantitatively profile 48 cancer-associated cytokine expression and compare the cytokine patterns among samples, including serum, plasma, tissue cell lysate.
Detection Range:
8 - 2000 pg/mL
Sensitivity:
5 - 10 pg/mL
Applicable Grid:
List of Applicable Cytokines
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | Adipo | CXCL16 | IGF | IL-6 | IL-22 | PDGF-BB | Adipo | CXCL16 | IGF | IL-6 | IL-22 | PDGF-BB |
| B | b-NGF | EGF | IGF-BP1 | IL-8 | IL-31 | PIGF-1 | b-NGF | EGF | IGF-BP1 | IL-8 | IL-31 | PIGF-1 |
| C | CCL27 | Eotaxin-3 | IL-1a | IL-10 | IP-10 | Rantes | CCL27 | Eotaxin-3 | IL-1a | IL-10 | IP-10 | Rantes |
| D | CTGF | FGFb | IL-1b | IL-11 | Leptin | Resistin | CTGF | FGFb | IL-1b | IL-11 | Leptin | Resistin |
| E | CXCL1 | G-CSF | IL-2 | IL-12 | MCP-1 | SCF | CXCL1 | G-CSF | IL-2 | IL-12 | MCP-1 | SCF |
| F | CXCL2 | GM-CSF | IL-3 | IL-13 | MIC-1a | TGFb | CXCL2 | GM-CSF | IL-3 | IL-13 | MIC-1a | TGFb |
| G | CXCL9 | ICAM-1 | IL-4 | IL-17a | Neuroserpin | TNFa | CXCL9 | ICAM-1 | IL-4 | IL-17a | Neuroserpin | TNFa |
| H | CXCL11 | IFNr | IL-5 | IL-17E | PAI-1 | VEGF | CXCL11 | IFNr | IL-5 | IL-17E | PAI-1 | VEGF |
Principle
The 96-well clear plate is divided into 2 sections, and each section has 6 columns for one sample. In each section, 48 specific cytokine capture antibodies are coated on 48 wells respectively. The sample, cell culture supernatants, cell lysates, tissue homogenates, serum, or plasma samples among others are incubated with the cytokine ELISA plate, and the captured cytokine proteins are subsequently detected with a cocktail of biotinylated detection antibodies. The test sample is allowed to react with a pair of antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The plate is further detected with HRP luminescent substrate or HRP substrate TMB. The level of expression for each specific cytokine is directly proportional to the luminescent or color intensity.
