Product Name Catalog # Price   Qty
Human Cytokine ELISA Plate Array I (Colorimetric) EA-4002 $946
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Characterization

Description(s):

Cytokines are signaling molecules that have critical roles in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity, and inflammation. Cytokines that are secreted from cells bind to cell-surface receptors, initiate the activation of signal transduction pathways and mediate cell to cell communication. The malfunction of cytokines leads to many diseases, including arthritis, acute and chronic liver disease, inflammatory bowel disease, cardiac-related diseases, and cancers. Cytokines are commonly working together in a biological or disease process. Therefore, the comprehensive analysis of the expression of multiple cytokines allows effective revealing of the underneath mechanism of cytokine action and the alteration leading to diseases. The Human Cytokine ELISA Plate Array I is a colormetric detection that allows you to monitor the abundance of 32 human cytokines simultaneously. This fast and sensitive assay can be used for quantitative comparison of these cytokines among different samples. 

Detection Range:
    8 - 2000 pg/mL

Sensitivity:
    5 pg/mL

 


Applicable Grid:

List of Applicable Cytokines

  1 2 3 4 5 6 7 8 9 10 11 12
A TNFa VEGF PDGF-BB IL-10 TNFa VEGF PDGF-BB IL-10 TNFa VEGF PDGF-BB IL-10
B IFNy EGF PIGF-1 FGFb IFNy EGF PLGF-1 FGFb IFNy EGF PLGF-1 FGFb
C G-CSF IL-6 b-NGF Leptin G-CSF IL-6 b-NGF Leptin G-CSF IL-6 b-NGF Leptin
D GM-CSF Resistin SCF IGF-1 GM-CSF Resistin SCF IGF-1 GM-CSF Resistin SCF IGF-1
E IL-1a PAI-1 MCP-1 TGF-b IL-1a PAI-1 MCP-1 TGF-b IL-1a PAI-1 MCP-1 TGF-b
F IL-8 IL-12 MIP-1a Adipo IL-8 IL-12 MIP-1a Adipo IL-8 IL-12 MIP-1a Adipo
G IP-10 IL-13 IL-2 IL-17a IP-10 IL-13 IL-2 IL-17a IP-10 IL-13 IL-2 IL-17a
H Rantes Eotaxin-3 IL-4 IL-1b Rantes Eotaxin-3 IL-4 IL-1b Rantes Eotaxin-3 IL-4 IL-1b

Principle

The 96-well clear plate is divided into 3 or 4 sections, and each section has 4 or 3 columns for one sample. In each section, 32 (Human Cytokine ELISA Plate Arrays) or 24 (Mouse Cytokine ELISA Plate Array) specific cytokine capture antibodies are coated on 32 or 24 wells respectively. The sample, cell culture supernatants, cell lysates, tissue homogenates, serum, or plasma samples among others are incubated with the cytokine ELISA plate, and the captured cytokine proteins are subsequently detected with a cocktail of biotinylated detection antibodies. The test sample is allowed to react with a pair of antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The plate is further detected with HRP luminescent substrate or HRP substrate TMB. The level of expression for each specific cytokine is directly proportional to the luminescent or color intensity.

Human color

Data

Analysis of Cytokine Protein Expression in TNFa-Treated and Untreated HeLa with Human Cytokine ELISA Plate Array. HeLa cells were starved for 24 hours with serum-free medium, subsequently treated the cells with and without 20ng/ul TNF for 16 hours. The serum-free conditioned media were incubated on the plate for 1 hour. After incubating with detection antibody mix and HRP, the plate was detected with TMB substrate by a plate reader.

 

Analysis of Cytokine Protein Expression in TNFa-Treated and Untreated HeLa with Human Cytokine ELISA Plate Array. HeLa cells were starved for 24 hours with serum-free medium, subsequently treated the cells with and without 20ng/ul TNF for 16 hours. The serum-free conditioned media were incubated on the plate for 1 hour. After incubating with detection antibody mix and HRP, the plate was detected with TMB substrate by a plate reader.

  
   

Literature

View user manual

 

Citations

Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cassetta, Luca, et al. Cancer Cell, 2019, doi:10.1016/j.ccell.2019.02.009.

Adipose tissue extract shows potential for wound healing: in vitro proliferation and migration of cell types contributing to wound healing in the presence of adipose tissue preparation and platelet rich plasma. Jenny F. López, Jertta-Riina Sarkanen, Outi Huttala, Ilkka S. Kaartinen, Hannu O. Kuokkanen, Timo Ylikomi. Cytotechnology, 2018, doi:10.1007/s10616-018-0211-y.

Priming hMSCs with a putative anti-cancer compound, myrtucommulone-a: a way to harness hMSC cytokine expression via modulating PI3K/Akt pathway? B Iskender, K Izgi, C Sakalar, H Canatan . Tumor Biology, First online: 03 September 2015.

ISG15 counteracts Listeria monocytogenes infection.L Radoshevich, F Impens, D Ribet, JJ Quereda.eLife. 2015; 4: e06848.Published online 2015 Aug 11. doi:  10.7554/eLife.06848.

Suppression of SERPINA1-albumin Complex Formation by Galectin-3 Overexpression Leads to Paracrine Growth Promotion of Chronic Myelogenous Leukemia Cells. Nakayama, Ryuko, Junya Kuroda, Nobuko Taniyama, Mio Yamamoto-Sugitani, Sayori Wada, Miki Kiyota, Shinsuke Mizutani, Yoshiaki Chinen, Yosuke Matsumoto, Hisao Nagoshi, Yuji Shimura, Tsutomu Kobayashi, Shigeo Horiike, Kenji Sato, and Masafumi Taniwaki. Leukemia Research 38.1 (2014): 103-08. Print.

Human Urine-Derived Stem Cells Alone or Genetically-Modified with FGF2 Improve Type 2 Diabetic Erectile Dysfunction in a Rat Model. B Ouyang, X Sun, D Han, S Chen, B Yao, Y Gao, J Bian etc. PLOS ONE, 2014 DOI:10.1371/journal.pone.0092825.

Extracellular DNA in pancreatic cancer promotes cell invasion and metastasis. Wen F, Shen A, Choi A, Gerner EW, Shi J. Cancer Res. 2013 Jul 15;73(14):4256-66. doi: 10.1158/0008-5472.CAN-12-3287. Epub 2013 May 30.

Radiotherapy-induced plasma cytokine changes and skin toxicity in breast cancer patients. Liu Z, Thomas V, Wright J, Takita C, Poitevien M, Abreu-Molnar D, Zhao W, Reis I, and Hu J.  Cancer Res., 2012 Apr;72: 4352.

Hartley, A., Wang, B., Mundade, R. et al. PRMT5-mediated methylation of YBX1 regulates NF-κB activity in colorectal cancer. Sci Rep 10, 15934 (2020). https://doi.org/10.1038/s41598-020-72942-3

EGR1 is a gatekeeper of inflammatory enhancers in human macrophages. Trizzino M, Zucco A, Deliard S, Wang F, Barbieri E, Veglia F, Gabrilovich D, Gardini A. Science Advances. 2021 Jan;7(3):eaaz8836. DOI: 10.1126/sciadv.aaz8836

3-N-butylphthalide protects against high-fat-diet-induced obesity in C57BL/6 mice and increases metabolism in lipid-accumulating cells. Kang-Yun Lu, Shinn-Zong Lin, Kingsley Theras Primus Dass, Wei-Ju Lin, Shih-Ping Liu, Horng-Jyh Harn. Biomedicine & Pharmacotherapy, Volume 139, 2021, 111687, ISSN 0753-3322, https://doi.org/10.1016/j.biopha.2021.111687.

Physoxia alters human mesenchymal stem cell secretome. Merkhan MM, Shephard MT, Forsyth NR.  Journal of Tissue Engineering. January 2021. doi:10.1177/20417314211056132