| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| Human Cytokine ELISA Plate Array II (Colorimetric) | EA-4010 | $946 |
Characterization
Description(s):Cytokines are signaling molecules that have critical roles in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity, and inflammation. Cytokines that are secreted from cells bind to cell-surface receptors, initiate the activation of signal transduction pathways and mediate cell to cell communication. The malfunction of cytokines leads to many diseases, including arthritis, acute and chronic liver disease, inflammatory bowel disease, cardiac-related diseases, and cancers. Cytokines are commonly working together in a biological or disease process. Therefore, the comprehensive analysis of the expression of multiple cytokines allows effective revealing of the underneath mechanism of cytokine action and the alteration leading to diseases. The Human Cytokine ELISA Plate Array II is a colorimetric detection that allows you to monitor the abundance of 32 human cytokines simultaneously. This fast and sensitive assay can be used for quantitative comparison of these cytokines among different samples.
Detection Range:
8 - 2000 pg/mL
Sensitivity:
5 - 10 pg/mL
Applicable Grid:
List of Applicable Cytokines
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | CNTF | IGF-BP1 | IL-21 | Neuroserpin | CNTF | IGF-BP1 | IL-21 | Neuroserpin | CNTF | IGF-BP1 | IL-21 | Neuroserpin |
| B | CCL21 | IL-3 | IL-22 | NOV | CCL21 | IL-3 | IL-22 | NOV | CCL21 | IL-3 | IL-22 | NOV |
| C | CTGF | IL-5 | IL-31 | CXCL12 | CTGF | IL-5 | IL-31 | CXCL12 | CTGF | IL-5 | IL-31 | CXCL12 |
| D | CXCL16 | IL-7 | CXCL11 | TACI | CXCL16 | IL-7 | CXCL11 | TACI | CXCL16 | IL-7 | CXCL11 | TACI |
| E | EG-VEGF | IL-9 | MIA-2 | TIMP-1 | EG-VEGF | IL-9 | MIA-2 | TIMP-1 | EG-VEGF | IL-9 | MIA-2 | TIMP-1 |
| F | CXCL1 | IL-11 | Midkine | TL-1A | CXCL1 | IL-11 | MIP-1a | Adipo | IL-8 | IL-12 | MIP-1a | Adipo |
| G | CXCCL2 | IL-17E | CXCL9 | TSLP | CXCCL2 | IL-17E | CXCL9 | TSLP | CXCCL2 | IL-17E | CXCL9 | TSLP |
| H | ICAM-1 | IL-20 | CXCL7 | TWEAK | ICAM-1 | IL-20 | CXCL7 | TWEAK | ICAM-1 | IL-20 | CXCL7 | TWEAK |
Principle
The 96-well clear plate is divided into 3 or 4 sections, and each section has 4 or 3 columns for one sample. In each section, 32 (Human Cytokine ELISA Plate Arrays) or 24 (Mouse Cytokine ELISA Plate Array) specific cytokine capture antibodies are coated on 32 or 24 wells respectively. The sample, cell culture supernatants, cell lysates, tissue homogenates, serum, or plasma samples among others are incubated with the cytokine ELISA plate, and the captured cytokine proteins are subsequently detected with a cocktail of biotinylated detection antibodies. The test sample is allowed to react with a pair of antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The plate is further detected with HRP luminescent substrate or HRP substrate TMB. The level of expression for each specific cytokine is directly proportional to the luminescent or color intensity.
Literature
View user manual
Citations
Human Urine-Derived Stem Cells Alone or Genetically-Modified with FGF2 Improve Type 2 Diabetic Erectile Dysfunction in a Rat Model. B Ouyang, X Sun, D Han, S Chen, B Yao, Y Gao, J Bian etc. PLOS ONE, 2014 DOI:10.1371/journal.pone.0092825.
Priming hMSCs with a putative anti-cancer compound, myrtucommulone-a: a way to harness hMSC cytokine expression via modulating PI3K/Akt pathway? B Iskender, K Izgi, C Sakalar, H Canatan . Tumor Biology, First online: 03 September 2015.
Suppression of SERPINA1-albumin Complex Formation by Galectin-3 Overexpression Leads to Paracrine Growth Promotion of Chronic Myelogenous Leukemia Cells. Nakayama, Ryuko, Junya Kuroda, Nobuko Taniyama, Mio Yamamoto-Sugitani, Sayori Wada, Miki Kiyota, Shinsuke Mizutani, Yoshiaki Chinen, Yosuke Matsumoto, Hisao Nagoshi, Yuji Shimura, Tsutomu Kobayashi, Shigeo Horiike, Kenji Sato, and Masafumi Taniwaki. Leukemia Research 38.1 (2014): 103-08. Print.
Human Urine-Derived Stem Cells Alone or Genetically-Modified with FGF2 Improve Type 2 Diabetic Erectile Dysfunction in a Rat Model. B Ouyang, X Sun, D Han, S Chen, B Yao, Y Gao, J Bian etc. PLOS ONE, 2014 DOI:10.1371/journal.pone.0092825.
ISG15 counteracts Listeria monocytogenes infection.L Radoshevich, F Impens, D Ribet, JJ Quereda.eLife. 2015; 4: e06848.Published online 2015 Aug 11. doi: 10.7554/eLife.06848.
Extracellular DNA in pancreatic cancer promotes cell invasion and metastasis. Wen F, Shen A, Choi A, Gerner EW, Shi J. Cancer Res. 2013 Jul 15;73(14):4256-66. doi: 10.1158/0008-5472.CAN-12-3287. Epub 2013 May 30.
Radiotherapy-induced plasma cytokine changes and skin toxicity in breast cancer patients. Liu Z, Thomas V, Wright J, Takita C, Poitevien M, Abreu-Molnar D, Zhao W, Reis I, and Hu J. Cancer Res., 2012 Apr;72: 4352.
Adipose tissue extract shows potential for wound healing: in vitro proliferation and migration of cell types contributing to wound healing in the presence of adipose tissue preparation and platelet rich plasma. Jenny F. López, Jertta-Riina Sarkanen, Outi Huttala, Ilkka S. Kaartinen, Hannu O. Kuokkanen, Timo Ylikomi. Cytotechnology, 2018, doi:10.1007/s10616-018-0211-y.
