Description(s):
Granulocyte colony-stimulating factor (G-CSF) is a colony-stimulating factor hormone. It is produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells and to stimulate the bone marrow to release them into the blood. It also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils, as well as monocytes. G-CSF influences monocyte functions in an anti-inflammatory way. The stimulation of monocytes with G-CSF results in an attenuation of LPS-induced release of IL-1, TNF-a, IL-12 and IL-18.
Detection Range:
- 16 - 1000 pg/mL
Sensitivity:
- 8 pg/mL
Principle
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The ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a mouse anti-human cytokine antibody for immobilization on the microtiter wells and goat anti-human cytokine antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules of the cytokine being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of the cytokine is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. |
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Data
Literature
View user manualCitations
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