Characterization
Description(s):Interleukins are a family of chemical messengers that can evoke a wide spectrum of responses in the body, including immune and inflammatory responses. Understanding the conditions that alter their expressions can be critical for unraveling the underlying mechanisms of diseases such as Rheumatoid Arthritis, Atherosclerosis, and a variety of cancers. Signosis’s Interleukin ELISA Plate Array can simultaneously analyze 21 interleukins. Each well is coated with a specific capture antibody to detect corresponding cytokine in the sample. The expression levels of these cytokines can be quantitatively compared between samples.
Detection Range:
8 - 2000 pg/mL
Sensitivity:
5 - 10 pg/mL
Applicable Grid:
List of Applicable Cytokines
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | IL-1a | IL-8 | IL-20 | IL-1a | IL-8 | IL-20 | IL-1a | IL-8 | IL-20 | IL-1a | IL-8 | IL-20 |
| B | IL-1b | IL-9 | IL-21 | IL-1b | IL-9 | IL-21 | IL-1b | IL-9 | IL-21 | IL-1b | IL-9 | IL-21 |
| C | IL-2 | IL-10 | IL-22 | IL-2 | IL-10 | IL-22 | IL-2 | IL-10 | IL-22 | IL-2 | IL-10 | IL-22 |
| D | IL-3 | IL-11 | IL-31 | IL-3 | IL-11 | IL-31 | IL-3 | IL-11 | IL-31 | IL-3 | IL-11 | IL-31 |
| E | IL-4 | IL-12 | IL-33 | IL-4 | IL-12 | IL-33 | IL-4 | IL-12 | IL-33 | IL-4 | IL-12 | IL-33 |
| F | IL-5 | IL-13 | Black | IL-5 | IL-13 | Black | IL-5 | IL-13 | Black | IL-5 | IL-13 | Black |
| G | IL-6 | IL-17a | Blank | IL-6 | IL-17a | Blank | IL-6 | IL-17a | Blank | IL-6 | IL-17a | Blank |
| H | IL-7 | IL-17E | Blank | IL-7 | IL-17E | Blank | IL-7 | IL-17E | Blank | IL-7 | IL-17E | Blank |
Principle
The 96-well clear plate is divided into 4 sections, and each section has 3 columns for one sample. In each section, 21 specific interleukin cytokine capture antibodies are coated on 24 wells respectively. The sample, cell culture supernatants, cell lysates, tissue homogenates, serum, or plasma samples among others are incubated with the cytokine ELISA plate, and the captured cytokine proteins are subsequently detected with a cocktail of biotinylated detection antibodies. The test sample is allowed to react with a pair of antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The plate is further detected with HRP luminescent substrate or HRP substrate TMB. The level of expression for each specific cytokine is directly proportional to the luminescent or color intensity.
