Description(s):
Matrix metalloproteinase-7 (MMP7) is an enzyme that breaks down macromolecules in the extracellular matrix. MMP7 is involved in various physiological processes including embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP7 plays a large role in tumor metastasis, since high levels of MMP7 facilitate cancer invasion by degrading extracellular matrix macromolecules and connective tissues. Overexpression of MMP7 is associated with malignant tumors in many tissues including the esophagus, stomach, colon, and liver.
Principle
The wells of the plate are coated with capture antibody specific to MMP7. In this assay, the test sample initially reacts with the solid phase capture antibody, resulting in MMP7 being bound to the well. The wells are then washed to remove unbound proteins, and biotin-linked antibodies are added to bind to the immobilized MMP7. After washing away the unbound antibodies, Streptavidin-HRP conjugate is added to form a complex with the antibody-bound MMP7. After incubation, the wells are washed to remove unbound Streptavidin-HRP conjugate. TMB substrate is then added and forms a blue color when the HRP-linked antibodies are detected. The reaction is then terminated with Stop Solution, which changes the color from blue to yellow. The MMP7 concentration in each well is directly proportional to its color intensity and can be quantified by measuring its optical density at 450 nm (OD450) in a microplate reader.
