Description(s):
Neuron specific enolase (NSE) is an enzyme that is expressed in developing neurons and glial cells. It is known to catalyze the production of phosphoenolpyruvate and is believed to possess neurotrophic activity for neurons. NSE could be a potentially useful biomarker for assessing neuronal damage and the prognosis of brain injury and can be measured in the blood and cerebrospinal fluid.
Principle
The wells of the plate are coated with capture antibody specific to NSE. In this assay, the test sample initially reacts with the solid phase capture antibody, resulting in NSE being bound to the well. The wells are then washed to remove unbound proteins, and biotin-linked antibodies are added to bind to the immobilized NSE. After washing away the unbound antibodies, Streptavidin-HRP conjugate is added to form a complex with the antibody-bound NSE. After incubation, the wells are washed to remove unbound Streptavidin-HRP conjugate. TMB substrate is then added and forms a blue color when the HRP-linked antibodies are detected. The reaction is then terminated with Stop Solution, which changes the color from blue to yellow. The NSE concentration in each well is directly proportional to its color intensity and can be quantified by measuring its optical density at 450 nm (OD450) in a microplate reader.
