Description(s):
S100B is a calcium-binding protein that is expressed primarily by astrocytes in the brain. This protein has a variety of functions including neurite extension, proliferation of melanoma cells, and microtubule assembly. Altered expression protein has been implicated in several neurological and neuroplastic diseases including Alzheimer disease, Down syndrome, and epilepsy. Furthermore, S100B levels are usually elevated in adults with nervous system damage, making this protein a potential marker for brain injury.
Principle
The wells of the plate are coated with capture antibody specific to S100B. In this assay, the test sample initially reacts with the solid phase capture antibody, resulting in S100B being bound to the well. The wells are then washed to remove unbound proteins, and biotin-linked antibodies are added to bind to the immobilized S100B. After washing away the unbound antibodies, Streptavidin-HRP conjugate is added to form a complex with the antibody-bound S100B. After incubation, the wells are washed to remove unbound Streptavidin-HRP conjugate. TMB substrate is then added and forms a blue color when the HRP-linked antibodies are detected. The reaction is then terminated with Stop Solution, which changes the color from blue to yellow. The S100B concentration in each well is directly proportional to its color intensity and can be quantified by measuring its optical density at 450 nm (OD450) in a microplate reader.
