Characterization
Description(s):T-Cells are a subset of lymphocytes that play a key role in the immune response. T-Cell Receptor (TCR) is a complex of integral membrane proteins that participates in the activation of T-Cells in response to antigen. This activation involves subunits of the receptor complex as well as other membrane-associated molecules that couple the extracellular liganding event to downstream signaling pathways such as the increase of inositol phosphates, the elevation of intracellular calcium levels and protein phosphorylation. TCR activation promotes a number of signaling cascades that ultimately determine cell fate through regulating cytokine production, cell survival, proliferation, and differentiation.
Signosis has developed a plate-based array for profiling 20+ Human T Cell-regulated genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.
Applicable Grid:
List of Applicable Genes
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | CD45 | JNK | PLCr | CD45 | JNK | PLCr | CD45 | JNK | PLCr | CD45 | JNK | PLCr |
| B | ELK | JUN | Raf1 | ELK | JUN | Raf1 | ELK | JUN | Raf1 | ELK | JUN | Raf1 |
| C | B-actin | LAT | Ras | B-actin | LAT | Ras | B-actin | LAT | Ras | B-actin | LAT | Ras |
| D | GADS | LCK | SLP76 | GADS | LCK | SLP76 | GADS | LCK | SLP76 | GADS | LCK | SLP76 |
| E | GRB2 | NFAT | SOS | GRB2 | NFAT | SOS | GRB2 | NFAT | SOS | GRB2 | NFAT | SOS |
| F | IL2 | NFkB | TCRz | IL2 | NFkB | TCRz | IL2 | NFkB | TCRz | IL2 | NFkB | TCRz |
| G | IP3R | p38 | Zap70 | IP3R | p38 | Zap70 | IP3R | p38 | Zap70 | IP3R | p38 | Zap70 |
| H | ITK | PKC-theta | blank | ITK | PKC-theta | blank | ITK | PKC-theta | blank | ITK | PKC-theta | blank |
Principle
Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.
Literature
View user manualCitations
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2. Embryonic Overexpression of Receptors for Advanced Glycation End-Products by Alveolar Epithelium Induces an Imbalance between Proliferation and Apoptosis. Stogsdill JA1, Stogsdill MP, Porter JL, Hancock JM, Robinson AB, Reynolds PR. Am J Respir Cell Mol Biol Vol 47, Iss. 1, pp 60–66, Jul 2012
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6. siRNA-mediated knockdown of aryl hydrocarbon receptor nuclear translocator 2 affects hypoxia-inducible factor-1 regulatory signaling and metabolism in human breast cancer cells. Edited by Judit Ovádi. Qin XY, Wei F, Yoshinaga J, Yonemoto J, Tanokura M, Sone H. FEBS Lett. 2011 Oct 20;585(20):3310-5.
7. Tasquinimod is an allosteric modulator of HDAC4 survival signaling within the compromised cancer microenvironment. Isaacs JT, Antony L, Dalrymple S, Brennen WN, Gerber S, Hammers H, Wissing MD, Kachhap SK, Luo J, Xing L,Bjork P, Olsson A, Björk A, Leanderson T. Cancer Res. 2012 Nov 13.
8. HIF-stabilizing bioactive glasses for directing MSC behaviour . MM Azevedo, O Tsigkou, R Nair, G Jell, J Jones… - Tissue Engineering, 2014
9.Nitration of the Tumor Suppressor Protein p53 at Tyrosine 327 Promotes p53 Oligomerization and Activation. Yakovlev VA, Bayden AS, Graves PR, Kellogg GE, Mikkelsen RB. Biochemistry., 2010 Jun 29;49(25):5331-9. doi: 10.1021/bi100564w.
10. Expression of Wnt3 activates Wnt/β-catenin pathway and promotes EMT-like phenotype intrastuzumab resistant HER2-overexpressing breast cancer cells. Vadgama JV, Wu Y, Ginther C, Kim J, Mosher N, Chung S, Slamon D. Mol Cancer Res. 2012 Oct 15.
11. A83-01 inhibits TGF-β-induced upregulation of Wnt3 and epithelial to mesenchymal transition in HER2-overexpressing breast cancer cells. Yanyuan Wu, Trinh Tran, Sami Dwabe, Marianna Sarkissyan, Juri Kim, Miguel Nava, Sheilah Clayton, Richard Pietras, Robin Farias-Eisner, Jaydutt V. Vadgama. Breast Cancer Research and Treatment. 2017 March 23. doi: 10.1007/s10549-017-4211-y
12. Conditionally induced RAGE expression by proximal airway epithelial cells in transgenic mice causes lung inflammation.B Garrett Bodine, Brock G Bennion, Emma Leatham, Felix R Jimenez, Alex J Wright, Zac R Jergensen,Connor J Erickson, Cameron M Jones, Jeff P Johnson, Steven M Knapp and Paul R Reynolds . Respiratory Research, 2014 (Published online)
13. Ablation of Doublecortin-Like Kinase 1 in the Colonic Epithelium Exacerbates Dextran Sulfate Sodium-Induced Colitis. D Qu, N Weygant, R May, P Chandrakesan. Plos One Published: August 18, 2015, DOI: 10.1371/journal.pone.0134212
