Characterization
Description(s):IRF3 and IRF7 are members of the interferon regulatory transcription factor (IRF) family, involving in antiviral defense, cell growth regulation, and immune activation. Latent cytoplasmic IRF-3 and IRF7 are activated and phosphorylated following virus infection or treatment with dsRNA, and translocate to the nucleus and bind to their DNA binding sequences. Even though IRF3 and IRF7 are the key activators of the alpha/beta IFN genes, the recent studies have demonstrated that IRF3 and IRF7 have distinct DNA binding properties and induce preferentially on different promoters. Because of the common and distinct biological features of IRF-3 and IRF-7, Signosis have developed both IRF3 and IRF7 ELISA kits respectively and a combined kit (48 wells for IRF3 and 48 wells for IRF7) with specific DNA binding sites and antibodies to distinguish the activation of IRF3 and IRF7 in various biological conditions.
Applicable Grid:
IRF ELISA Kits Available:
Principle
TF ELISA kit is high sensitive and specific assay with a simple and optimized procedure. The 96-well (8X12 strip) clear plate is pre-immobilized with the TF consensus sequencing oligo. The activated TF in nuclear extract or the whole cell lysate is added in the well and binds to the oligo. The activated TF is detected with a specific antibody against the subunit and a HRP conjugated secondary antibody. The assay utilizes colorimetric detection method, which can be easily measured by spectrophotometry.
Data
The 293 cells were treated with 20ng/mlIFNg and 10ng/ml poly(I/C) for 8 hours. The nuclear extracts were prepared and subjucted to IRF7 ELISA kit
