Description(s):
Established from pleural effusion of 53 year old female with chronic myelogenous leukaemia in terminal blast crisis. Population highly undifferentiated and of the granulocytic series. Can be used as a highly sensitive target for the in vitro natural killer assay. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series.
Cell Line Origin
Human Caucasian chronic myelogenous leukaemia
Principle
|
Cell line name |
K562 |
| Catalog number | PC-007 |
| Species | Human |
| Tissue | Bone marrow |
| Description | Tissue: Bone marrow |
| Morphology/property | Lymphoblast/suspension |
| Growth | |
| Culture condition | Complete growth medium: In 450mL of RPMI, add 50mL FBS (10% final) and 5mL Penicillin/Streptomycin (1% final). |
| Subculturing | Culture at 1 X 105 viable cells/mL. Subculture at 1X106 cells/mL. |
| Medium Renewal: Every 2 to 3 days | |
| Hazard | Handle as Biosafety Level 1 containment |
| Storage Conditions | Liquid nitrogen |
| Freezing medium | Complete growth medium + 10% DMSO |
