| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| Lysosomal Stress TF Activation Profiling Plate Array with Nuclear Extraction Kit | FA-1012-NE | $971 |
Characterization
Description(s):The lysosome plays a key role in cellular homeostasis by controlling both cellular clearance and energy production to respond to environmental cues. It is well known that transcription factor EB (TFEB) and mTOR complex 1 (mTORC1) are master regulators of lysosomal biogenesis. Under starvation, cells initiate the process of autophagy and increase the capacity of the lysosome by the lysosome stress response. mTORC1 dissociates from the lysosome and becomes inactivated, leading to dephosphorylation of TFEB and autophagy. Dephosphorylated TFEB enters the nucleus, binds to the enhancer element CLEAR, and activates transcription of genes related to the lysosome as well as autophagy. The lysosome stress response is closely related to various diseases such as Huntington’s disease, liver disease and lysosomal storage diseases including Pompe disease. Recently, more TFs have been reported to be associated with lysosome stress, such as p53, FOXO1, PPAR, Stat3, NFkB, HIF, HSF and MITF.
Applicable Grid:
List of Applicable TFs:
| 1 | 2 | 3 |
4 |
5 |
6 |
7 | 8 | 9 | 10 |
11 |
12 | |
| A | FOXO1 | FOXO1 | FOXO1 | FOXO1 | FOXO1 | FOXO1 | FOXO1 | FOXO1 | FOXO1 | FOXO1 | FOXO1 | FOXO1 |
| B | HIF | HIF | HIF | HIF | HIF | HIF | HIF | HIF | HIF | HIF | HIF | HIF |
| C | HSF | HSF | HSF | HSF | HSF | HSF | HSF | HSF | HSF | HSF | HSF | HSF |
| D | MITF | MITF | MITF | MITF | MITF | MITF | MITF | MITF | MITF | MITF | MITF | MITF |
| E | NFkB | NFkB | NFkB | NFkB | NFkB | NFkB | NFkB | NFkB | NFkB | NFkB | NFkB | NFkB |
| F | p53 | p53 | p53 | p53 | p53 | p53 | p53 | p53 | p53 | p53 | p53 | p53 |
| G | PPAR | PPAR | PPAR | PPAR | PPAR | PPAR | PPAR | PPAR | PPAR | PPAR | PPAR | PPAR |
| H | STAT3 | STAT3 | STAT3 | STAT3 | STAT3 | STAT3 | STAT3 | STAT3 | STAT3 | STAT3 | STAT3 | STAT3 |
Principle
ignosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.
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