Description(s):
Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
Cell Line Origin
Human Caucasian breast adenocarcinoma
Principle
| Cell line name | MCF7 |
| Catalog number | PC-019 |
| Species | Human |
| Tissue | Mammary gland, breast; derived from metastatic site: pleural effusion |
| Description | Tissue: mammary gland, epithelial |
| Morphology/property | Epithelial-like/adherent |
| Growth | Continuous culture, grown as monolayer |
| Culture condition | Complete growth medium: In 450mL of EMEM, add 50mL FBS (10% final) and 5mL Penicillin/Streptomycin (1% final). |
| Subculturing | Subcultivation Ratio: 1:3 to 1:6 weekly |
| Medium Renewal: 2 to 3 times per week | |
| Hazard | Handle as Biosafety Level 1 containment |
| Storage Conditions | Liquid nitrogen |
| Freezing medium | Complete growth medium + 10% DMSO |
