How to order this kit:

Step 1: choose miRNA Northern Blot

Step 2: choose oligo for miRNA of interest

Description(s):

Expression of miRNAs is highly regulated and tissue-specific.  Many of miRNAs are differentially expressed in human cancers.  Microarray profiling of miRNA expression leads to identification of differentially expressed miRNA in cancers.  MiRNA expression, tissue distribution, and microarray data validation often need northern blot analysis, with which the difference can be directly visualized, mature miRNA can be differentiated from its precursor, and the expression level of miRNA can be compared with internal controls such as U6 RNA.  Signosis miRNA northern blot assay kits provide a non-radioactive, simple, and highly sensitive tool to conduct the assay.  These kits contain all the components needed for northern blot analysis.  More importantly, these kits do not need the use of isotope and probe preparation. Reagents for RNA transfer, hybridization, and detection are included in the kits.

Note: Our miRNA Northern Blot kits no longer come with precast gels. It is highly recommended for our customers to provide their own gels instead. Freshly made gels can be altered and refined to better suit our customer's experiments. Instructions on creating your own gel are also included in the manual which can be used as a guideline. If kits with precast gels are still desired, please contact us for pricing and availability.

Principle

RNA samples are separated through gel electrophoresis and transferred onto a membrane. Expression of a specific miRNA is detected with a biotin-labeled probe. This regular Northern blot assay is suitable to most moderate to high expressors of miRNA. High sensitive Northern blot (NB-1001 and HP-XXXX) is needed to analyze low expressors of miRNA. The biotin-labeled probes contain two moieties – complementary sequence of the miRNA and a tag sequence. The tag sequence is then detected by an amplifier enriched with biotin molecules.

Data

Figure 1. miRNA northern blot validation of array results in human brain and liver with miR-122a and miR-125b probes.

Literature

View user manual