How to order this kit: 


Step 1: choose miRNA plate

Product Name Catalog # Price   Qty
miRNA Plate Assay Kit (Use with MO-XXXX) MA-0101 $545
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Step 2: choose oligo for miRNA of interest

Product Name Catalog # Price   Qty
miR-196a-5p MO-0077 $143
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miR-197-3p MO-0078 $143
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miR-198 MO-0079 $143
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miR-199a-5p MO-0080 $143
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miR-199a-5p MO-0081 $143
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miR-199b-5p MO-0082 $143
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miR-200a-3p MO-0083 $143
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miR-202-5p MO-0084 $143
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miR-203a MO-0085 $143
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miR-204-5p MO-0086 $143
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miR-205-5p MO-0087 $143
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miR-206 MO-0088 $143
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miR-210-3p MO-0089 $143
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miR-214-3p MO-0090 $143
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miR-215-5p MO-0091 $143
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miR-216-5p MO-0092 $143
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miR-218-5p MO-0093 $143
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miR-219 MO-0094 $143
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miR-221-3p MO-0095 $143
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miR-222-3p MO-0096 $143
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miR-146b-5p MO-0097 $143
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miR-224-5p MO-0098 $143
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miR-296-5p MO-0100 $143
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miR-329 MO-0101 $143
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miR-342-3p MO-0102 $143
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miR-372-3p MO-0104 $143
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miR-373-5p MO-0105 $143
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miR-375 MO-0106 $143
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miR-488-5p MO-0107 $143
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miR-497-5p MO-0501 $143
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miR-499-5p MO-0502 $143
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miR-507 MO-0503 $143
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miR-369-3p MO-0504 $143
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miR-369-5p MO-0505 $143
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miR-720 MO-0506 $143
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miR-213 (miR-181a) MO-0200 $143
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miR-223-3p MO-0507 $143
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miR-609 MO-0508 $143
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miR-760 MO-0509 $143
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sno234 MO-0510 $143
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miR-124a-3p MO-0037 $130
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RNU48 MO-0511 $143
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U6 MO-0512 $143
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let-7b-5p MO-0521 $143
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let-7e-5p MO-0522 $143
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let-7c-5p MO-0523 $143
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miR-125a-5p MO-0038 $130
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let-7d-5p MO-0524 $143
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let-7f-5p MO-0525 $143
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let-7g-5p MO-0526 $143
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let-7i-5p MO-0527 $143
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miR-125b-5p MO-0039 $130
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miR-29b-3p MO-0528 $143
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miR-29c-3p MO-0529 $143
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miR-181b-5p MO-0530 $143
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miR-126-3p MO-0040 $130
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miR-196b-5p MO-0531 $143
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miR-200c-3p MO-0532 $143
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mir-376c-3p (miR-368) MO-0533 $143
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miR-100-5p MO-0534 $143
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miR-127-5p MO-0535 $143
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miR-128a-3p MO-0041 $130
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miR-142-5p MO-0536 $143
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miR-31-5p MO-0537 $143
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miR-144-3p MO-0538 $143
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miR-128b-3p MO-0042 $130
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miR-152-3p MO-0539 $143
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miR-184 MO-0540 $143
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miR-337-3p MO-0541 $143
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miR-338 MO-0542 $143
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miR-131 MO-0043 $130
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miR-345-5p MO-0543 $143
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miR-371a-3p MO-0544 $143
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miR-96-5p MO-0545 $143
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miR-193a-3p MO-0546 $143
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miR-132-3p MO-0044 $130
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miR-193a-5p MO-0547 $143
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miR-133a-3p MO-0045 $130
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miR-133b MO-0046 $130
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miR-134-5p MO-0047 $130
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miR-135b-5p MO-0048 $130
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miR-137 MO-0049 $130
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miR-140-5p MO-0050 $130
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let-7a-5p MO-0001 $130
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miR-141-3p MO-0051 $130
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miR-1 MO-0002 $130
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miR-142-3p MO-0052 $130
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miR-7-5p MO-0003 $130
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miR-9-5p MO-0004 $130
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miR-136-5p MO-0053 $130
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miR-30a-5p MO-0005 $130
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miR-143-3p MO-0054 $130
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miR-10a-5p MO-0006 $130
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miR-145-5p MO-0055 $130
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miR-15a-5p MO-0007 $130
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miR-16-5p MO-0008 $130
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miR-146a-5p MO-0056 $130
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miR-17-3p MO-0009 $130
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miR-148a-3p MO-0057 $130
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miR-17-5p MO-0010 $130
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miR-18a-5p MO-0011 $130
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miR-149-5p MO-0058 $130
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miR-19a-3p MO-0012 $130
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miR-150-5p MO-0059 $130
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miR-20a-5p MO-0013 $130
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miR-151-3p MO-0060 $130
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miR-21-5p MO-0014 $130
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miR-22-3p MO-0015 $130
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miR-153-3p MO-0061 $130
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miR-23a-3p MO-0016 $130
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miR-154-5p MO-0062 $130
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miR-24-3p MO-0017 $130
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miR-155-5p MO-0063 $130
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miR-25-3p MO-0018 $130
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miR-26a-5p MO-0019 $130
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miR-181a-5p MO-0064 $130
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miR-27a-3p MO-0020 $130
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miR-181c-5p MO-0065 $130
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miR-28-5p MO-0021 $130
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miR-181d-5p MO-0066 $130
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miR-29a-3p MO-0022 $130
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miR-30a-3p MO-0023 $130
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miR-182-5p MO-0067 $130
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miR-30b-5p MO-0024 $130
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miR-183-5p MO-0068 $130
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miR-92-3p MO-0025 $130
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miR-185-5p MO-0069 $130
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miR-92b-3p MO-0026 $130
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miR-93-5p MO-0027 $130
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miR-186-5p MO-0070 $130
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miR-95-3p MO-0028 $130
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miR-188-5p MO-0071 $130
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miR-99a-5p MO-0029 $130
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miR-190-5p MO-0072 $130
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miR-30c-5p MO-0030 $130
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miR-101-3p MO-0031 $130
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miR-191-5p MO-0073 $130
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miR-103-3p MO-0032 $130
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miR-192-5p MO-0074 $130
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miR-106a-5p MO-0033 $130
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miR-194-5p MO-0075 $130
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miR-106b-5p MO-0034 $130
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miR-107 MO-0035 $130
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miR-195-5p MO-0076 $130
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miR-122a-5p MO-0036 $130
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Characterization

Description(s):

MicroRNAs (miRNAs) are small noncoding RNAs that control gene expression at the posttranscriptional level through selectively binding to complementary messenger RNA sequences.  Approximately 30% of mammalian genes are regulated by these small RNA molecules, which affect a variety of biological functions, including development, cell differentiation, proliferation, apoptosis, and maintenance of stemness and imprinting. 
Northern blotting has been the most commonly used method for analyzing individual miRNAs, although recently a number of new approaches have been developed, such as the related real-time PCR and invader assays. Low sensitivity and poor throughput are the main issues of Northern blot analysis. To resolve this problem,  Signosis has developed the miRNA plate assay kits. The kits are highly sensitive, and the biochemical conversion of miRNA molecules into cDNAs is not needed. The procedure of the assay is as simple as incubation and wash.

To analyze a  miRNA with the plate assay, you need to purchase the common component miRNA plate assay kit (MA-0101) and  a specific oligo mix (MO-XXXX) corresponding to the miRNA.


Applicable Grid:

Oligos avaible for miRNA Plate Assay Kit:

let-7a-5p miR-22-3p miR-99a-5p miR-135b-5p miR-181a-5p miR-198 miR-223-3p miR-720
let-7b-5p miR-23a-3p miR-100-5p miR-136-5p miR-181b-5p miR-199a-5p miR-224-5p miR-760
let-7c-5p miR-24-3p miR-101-3p miR-137 miR-181c-5p miR-199a*-3p miR-296-5p  
let-7d-5p miR-25-3p miR-103-3p miR-140-5p miR-181d-5p miR-199b-5p miR-329  
let-7e-5p miR-26a-5p miR-106a-5p miR-141-3p miR-182-5p miR-200a-3p miR-337-3p  
let-7f-5p miR-27a-3p miR-106b-5p miR-142-3p miR-183-5p miR-200c-3p miR-338  
let-7g-5p miR-28-5p miR-107 miR-142-5p miR-184 miR-202-5p miR-342-3p  
let-7i-5p miR-29a-3p miR-122a-5p miR-143-3p miR-185-5p miR-203a miR-345  
miR-1 miR-29b-3p miR-124a-3p miR-144 miR-186-5p miR-204-5p mir-376c-3p (miR-368)  
miR-7-5p miR-29c-3p miR-125a-5p miR-145-5p miR-188-5p miR-205-5p miR-369-3p  
miR-9-5p miR-30a-3p miR-125b-5p miR-146a-5p miR-190-5p miR-206 miR-369-5p  
miR-10a-5p miR-30a-5p miR-126-3p miR-146b-5p miR-191-5p miR-210-3p miR-371  
miR-15a-5p miR-30b-5p miR-127 miR-148a-3p miR-192-5p miR-213 (miR-181a) miR-372-3p  
miR-16-5p miR-30c-5p miR-128a-3p miR-149-5p miR-193a-3p miR-214-3p miR-373-5p  
miR-17-3p miR-31-5p miR-128b-3p miR-150-5p miR-193a-5p miR-215-5p miR-375  
miR-17-5p miR-92-3p miR-131 miR-151-3p miR-194-5p miR-216-5p miR-488-5p  
miR-18a-5p miR-92b-3p miR-132-3p miR-153-3p miR-195-5p miR-218-5p miR-497  
miR-19a-3p miR-93-5p miR-133a-3p miR-152 miR-196a-5p miR-219 miR-499-5p  
miR-20a-5p miR-95-3p miR-133b miR-154-5p miR-196b-5p miR-221-3p miR-507  
miR-21-5p miR-96 miR-134-5p miR-155-5p miR-197-3p miR-222-3p miR-609  

Principle

In the proprietary miRNA plate assay, one miRNA molecule is flanked by a capture oligo and a biotinated detection oligo through two bridges oligos. One of the bridge oligos is partially hybridized with the miRNA molecule and the capture oligo and another with the miRNA and the detection oligo. The hybrid is immobilized onto plate through hybridization with an immobilized oligo and detected by a streptavidin-HRP conjugate and chemiluminecscent substrate. This hybrid structure is sensitive to the sequence of the miRNA molecule. One nucleotide difference will prevent the formation of the hybrid and therefore miRNA isoform can be differentiated, which normally is hard to tackle with Northern blot. In addition, the sensitivity of the assay is higher than miRNA Northern blot assay.


Data



miRNA plate assay. Both miR-133 and miR-1 are preferentially expressed in cardiac and skeletal muscles and play essential roles in differentiation, proliferation and apoptosis of cardiac cells. Dysregulated miRNA expression has been correlated to diseased hearts in human patients and therefore, they are with great potentials as diagnostic markers and therapeutic targets for cardiovascular disease. (A) Schematice diagram of miRNA plate assay. (B) Expression of miR-133 and miR-1 was analyzed with 5ug of total RNA prepared from human sckeletal muscle, heart, and liver through Northern blot (top) and miRNA plat assay (bottom). (C) Detection of miR-133 expression was compared with Northern blot (top) and miRNA plate assay (bottom).
 

Literature

View user manual

Citations

miR-195
 
Silencing of miR-195 reduces diabetic cardiomyopathy in C57BL/6 mice.D Zheng, J Ma, Y Yu, M Li, R Ni, G Wang, R Chen, J Li… - Diabetologia, 2015 DOI10.1007/S00125-015-3622-8


miR-128
 
A high impact peer reviewed journal publishing experimental and theoretical work across the breadth of nanoscience and nanotechnology.YL Lo, HL Chou, ZX Liao, SJH Huang, YS Liu, JH Ke… - Nanoscale, 2015


miR -Let -7a, let-7b
 
Epigenetic dimension of oxygen radical injury in spermatogonial epithelial cells. PK Mishra, N Bunkar, GV Raghuram, NK Khare. Reprod Toxicol. 2015 Apr;52:40-56. doi: 10.1016/j.reprotox.2015.02.006. Epub 2015 Feb 14.

miR-29b
 
MicroRNA-29b promotes high-fat diet-stimulated endothelial permeability and apoptosis in apoE knock-out mice by down-regulating MT1 expression. HQ Zhu, LY Dong, Q Zhou, H Wang, Y Wang - Int J Cardiol. 2014 Oct 20;176(3):764-70. doi: 10.1016/j.ijcard.2014.07.095. Epub 2014 Aug 1. 

miR-195
 
MicroRNA-195 promotes palmitate-induced apoptosis in cardiomyocytes by down-regulating Sirt1. Zhu H, Yang Y, Wang Y, Li J, Schiller PW, Peng T.  Cardiovasc Res. 2011 Oct 1;92(1):75-84.
 
miR-1145
 
Colonic miRNA Expression/Secretion, Regulated by Intestinal Epithelial PepT1, Plays an Important Role in Cell-to-Cell Communication during Colitis. Ayyadurai S, Charania MA, Xiao B, Viennois E, Zhang Y, Merlin D. PLoS One. 2014 Feb 19;9(2):e87614. doi: 10.1371/journal.pone.0087614. eCollection 2014.
 
miRNA-1
 
MicroRNA-1 prevents high-fat diet-induced endothelial permeability in apoE knock-out mice. Wang H, Zhu HQ, Wang F, Zhou Q, Gui SY, Wang Y. Mol Cell Biochem. 2013 Jun;378(1-2):153-9.