| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| Mitochondrial UPR TF Activation Profiling Plate Array with Nuclear Extraction Kit | FA-1010-NE | $971 |
Characterization
Description(s):Protein folding within the mitochondria faces challenges from organelle structure, ROS production from mitochondria’s role in bioenergetics metabolism, and assembly of complex macromolecules in stoichiometric manner. To maintain protein homeostasis, a stress response known as Mitochondrial Unfolded Protein Response (UPRmt) upregulates chaperones gene expression and increases mitochondrial folding capacity during stress. This prevents deleterious protein aggregation and maintains proper protein folding.
Signosis, Inc. has developed the Mitochondrial UPR TF Activation Profiling Plate Array, which can be used to monitor 16 mitochondrial UPR related TFs simultaneously:AP1, ATF4, C/EBP, CHOP, E2F1, FOXO3, HIF, HSF, MEF2, NFkB, NRF1, NRF2/ARE, p53, SATB, TFEB and XBP.
Applicable Grid:
List of Applicable TFs:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | AP1 | MEF2 | AP1 | MEF2 | AP1 | MEF2 | AP1 | MEF2 | AP1 | MEF2 | AP1 | MEF2 |
| B | ATF4 | NFkB | ATF4 | NFkB | ATF4 | NFkB | ATF4 | NFkB | ATF4 | NFkB | ATF4 | NFkB |
| C | C/EBP | NRF1 | C/EBP | NRF1 | C/EBP | NRF1 | C/EBP | NRF1 | C/EBP | NRF1 | C/EBP | NRF1 |
| D | CHOP | NRF2/ARE | CHOP | NRF2/ARE | CHOP | NRF2/ARE | CHOP | NRF2/ARE | CHOP | NRF2/ARE | CHOP | NRF2/ARE |
| E | E2F1 | p53 | E2F1 | p53 | E2F1 | p53 | E2F1 | p53 | E2F1 | p53 | E2F1 | p53 |
| F | FOXO3 | SATB | FOXO3 | SATB | FOXO3 | SATB | FOXO3 | SATB | FOXO3 | SATB | FOXO3 | SATB |
| G | HIF | TFEB | HIF | TFEB | HIF | TFEB | HIF | TFEB | HIF | TFEB | HIF | TFEB |
| H | HSF | XBP | HSF | XBP | HSF | XBP | HSF | XBP | HSF | XBP | HSF | XBP |
Principle
Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.
Literature
View user manualCitations
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