Product Name Catalog # Price   Qty
Mouse Cytokine cDNA Plate Array AP-1141 $648
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Characterization

Description(s):

Cytokines are essential molecules that play crucial roles in many biological functions, including viral infection, inflammation, immunity, and hematopoiesis. Cytokines are produced by a variety of cell types in response to different stimuli. In addition, the expression of cytokine genes appears to be regulated by complex mechanism. Expression of one cytokine gene could be regulated by other cytokines. Dysregulation of cytokine gene expression may be caused by chromosomal alterations or by infection of viruses that induce activation or inactivation of the expression machinery. Therefore, expression profiling of cytokine genes could provide a useful approach to uncover the mechanism underlying their regulation. 

Signosis has developed a plate-based array for profiling 20+ Cytokine genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.

 




Applicable Grid:

List of Applicable Genes

  1 2 3 4 5 6 7 8 9 10 11 12
A 18S rRNA IL-13 IL-4 18S rRNA IL-13 IL-4 18S rRNA IL-13 IL-4 18S rRNA IL-13 IL-4
B Eotaxin IL-14 IL-5 Eotaxin IL-14 IL-5 Eotaxin IL-14 IL-5 Eotaxin IL-14 IL-5
C GAPDH  IL-15 IL6  GAPDH  IL-15 IL6  GAPDH  IL-15 IL6  GAPDH  IL-15 IL6 
D IFNb IL-16 IL-7 IFNb IL-16 IL-7 IFNb IL-16 IL-7 IFNb IL-16 IL-7
E IFNg IL-17 TGFa IFNg IL-17 TGFa IFNg IL-17 TGFa IFNg IL-17 TGFa
F IL-10 IL1A  TGFb IL-10 IL1A  TGFb IL-10 IL1A  TGFb IL-10 IL1A  TGFb
G IL11 IL2 TNFa IL11 IL2 TNFa IL11 IL2 TNFa IL11 IL2 TNFa
H IL-12 IL-3 VEGFC IL-12 IL-3 VEGFC IL-12 IL-3 VEGFC IL-12 IL-3 VEGFC

Principle

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.

Data


Mouse cytokine cDNA plate array analysis.  NIH3T3 cells were treated with (red bars) or without (light blue) 10ng/ml TNF for 90 min.  Cells were collected for profiling 23 genes with the cDNA plate array assay.

  
 

Literature

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Mouse Cytokine cDNA Plate Array AP-1141
 
Microglia RAGE exacerbates the progression of neurodegeneration within the SOD1G93A murine model of amyotrophic lateral sclerosis in a sex-dependent manner. MacLean, M., Juranek, J., Cuddapah, S. et al. J Neuroinflammation 18, 139 (2021). https://doi.org/10.1186/s12974-021-02191-2