Product Name Catalog # Price   Qty
Mouse Cytokine ELISA Plate Array I (Chemiluminescence) EA-4003 $817
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Characterization

Description(s):

Cytokines are signaling molecules that have critical roles in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity, and inflammation. Cytokines that are secreted from cells bind to cell-surface receptors, initiate the activation of signal transduction pathways and mediate cell to cell communication. The malfunction of cytokines leads to many diseases, including arthritis, acute and chronic liver disease, inflammatory bowel disease, cardiac-related diseases, and cancers. Cytokines are commonly working together in a biological or disease process. Therefore, the comprehensive analysis of the expression of multiple cytokines allows effectively revealing the underneath mechanism of cytokine action and the alteration leading to diseases. The Mouse Cytokine ELISA Plate Array is a chemiluminescent detection that allows you to monitor the abundance of 24 mouse cytokines simultaneously. This fast and sensitive assay can be used for quantitative comparison of these cytokines among different samples.

Detection Range:
    2 - 2000 pg/mL

Sensitivity:
    1 pg/mL


Applicable Grid:

List of Applicable Cytokines

  1 2 3 4 5 6 7 8 9 10 11 12
A TNFa IL-1a PDGF-BB TNFa IL-1a PDGF-BB TNFa IL-1a PDGF-BB TNFa IL-1a PDGF-BB
B IGF-1 IL-1b b-NGF IGF-1 IL-1b b-NGF IGF-1 IL-1b b-NGF IGF-1 IL-1b b-NGF
C VEGF G-CSF IL-17A VEGF G-CSF IL-17A VEGF G-CSF IL-17A VEGF G-CSF IL-17A
D IL-6 GM-CSF IL-2 IL-6 GM-CSF IL-2 IL-6 GM-CSF IL-2 IL-6 GM-CSF IL-2
E FGFb MCP-1 IL-4 FGFb MCP-1 IL-4 FGFb MCP-1 IL-4 FGFb MCP-1 IL-4
F IFNy MIP-1a IL-10 IFNy MIP-1a IL-10 IFNy MIP-1a IL-10 IFNy MIP-1a IL-10
G EGF SCF Resistin  EGF SCF Resistin  EGF SCF Resistin  EGF SCF Resistin 
H Leptin Rantes IL-12 Leptin Rantes IL-12 Leptin Rantes IL-12 Leptin Rantes IL-12

Principle

The 96-well white plate is divided into 4 sections, and each section has 3 strips for one sample. In each section, 24 of specific cytokine capture antibodies are coated on 24 wells respectively, and one well without coating any antibody is used as a blank well.  The sample, such as cell culture supernatants, cell lysates, tissue homogenates, serum, or plasma samples is incubated with cytokine ELISA plate, and the captured cytokine proteins are subsequently detected with a cocktail of biotinylated detection antibodies. The test sample is allowed to react with pairs of two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The plate is further detected with HRP luminescent substrate. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The level of expression for each specific cytokine is directly proportional to the luminescent intensity.

Data

 

 

 

 

 

 

 

 

 

 


Analysis of Cytokine Protein Expression in TNFa-Treated and Untreated NIH3T3 with Mouse Cytokine ELISA Plate Array. NIH/3T3 cells were starved for 24 hours with serum-free medium, subsequently treated the cells with and without 20ng/ul TNF for 16 hours. The serum-free conditioned media were incubated on the plate for 1 hour. After incubating with detection antibody mix and HRP, the plate was detected with chemilumincent substrate by a plate reader.

  

Literature

View user manual

 

Citations

Electrical stimulation with periodic alternating intervals stimulates neuronal cells to produce neurotrophins and cytokines through activation of mitogen-activated protein kinase pathways. K Yamamoto, T Yamamoto, K Honjo, H Ichioka. European Journal of Oral Sciences. Article first published online: 29 OCT 2015. DOI: 10.1111/eos.12224.

Parasites alter the pathological phenotype of lupus nephritis. K Miyake, K Adachi, M Watanabe, Y Sasatomi etc.   Autoimmunity. 2014 Dec;47(8):538-47. doi: 10.3109/08916934.2014.929669. Epub 2014 Jun 24.

No requirement of interlukine-1 for long-term potentiation in the anterior cingulate cortex of adult mice. Lu, Jing-Shan, et al. Molecular Pain, vol. 14, 2018, p. 174480691876579., doi:10.1177/1744806918765799.

Block of A1 astrocyte conversion by microglia is neuroprotective in models of Parkinson’s disease. Yun, Seung Pil, et al.  Nature Medicine, 2018, doi:10.1038/s41591-018-0051-5.

Compromised Astrocyte Function and Survival Negatively Impact Neurons in Infantile Neuronal Ceroid Lipofuscinosis. Lange, Jenny, et al. Acta Neuropathologica Communications, vol. 6, no. 1, 2018, doi:10.1186/s40478-018-0575-4.

Reduced AMPK activation and increased HCAR activation drive anti-inflammatory response and neuroprotection in glaucoma. Harun-Or-Rashid, Mohammad, and Denise M. Inman.  Journal of Neuroinflammation, vol. 15, no. 1, 2018, doi:10.1186/s12974-018-1346-7.

Mine-site derived particulate matter exposure exacerbates neurological and pulmonary inflammatory outcomes in an autoimmune mouse model. Alexis Wilson, Carmen A. Velasco, Guy W. Herbert, Selita N. Lucas, Bethany N. Sanchez, José M. Cerrato, Michael Spilde, Quan-Zhen Li, Matthew J. Campen & Katherine E. Zychowski (2021), Journal of Toxicology and Environmental Health, Part A, DOI: 10.1080/15287394.2021.1891488

3-N-butylphthalide protects against high-fat-diet-induced obesity in C57BL/6 mice and increases metabolism in lipid-accumulating cells. Kang-Yun Lu, Shinn-Zong Lin, Kingsley Theras Primus Dass, Wei-Ju Lin, Shih-Ping Liu, Horng-Jyh Harn. Biomedicine & Pharmacotherapy, Volume 139, 2021, 111687, ISSN 0753-3322, https://doi.org/10.1016/j.biopha.2021.111687.