| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| Mouse Cytokine ELISA Plate Array II (Chemiluminescence) | EA-4021 | $817 |
Characterization
Description(s):Cytokines are signaling molecules that have critical roles in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity, and inflammation. Cytokines that are secreted from cells bind to cell-surface receptors, initiate the activation of signal transduction pathways and mediate cell to cell communication. The malfunction of cytokines leads to many diseases, including arthritis, acute and chronic liver disease, inflammatory bowel disease, cardiac-related diseases, and cancers. Cytokines are commonly working together in a biological or disease process. Therefore, the comprehensive analysis of the expression of multiple cytokines allows effectively revealing the underneath mechanism of cytokine action and the alteration leading to diseases. The Mouse Cytokine ELISA Plate Array is a chemiluminescent detection that allows you to monitor the abundance of 32 mouse cytokines simultaneously. This fast and sensitive assay can be used for quantitative comparison of these cytokines among different samples.
Applicable Grid:
List of Applicable Cytokines
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | TNFa | IL-1a | PDGF-BB | IL-5 | TNFa | IL-1a | PDGF-BB | IL-5 | TNFa | IL-1a | PDGF-BB | IL-5 |
| B | IGF-1 | IL-1b | b-NGF | IL-15 | IGF-1 | IL-1b | b-NGF | IL-15 | IGF-1 | IL-1b | b-NGF | IL-15 |
| C | VEGF | G-CSF | IL-17A | IL-23 | VEGF | G-CSF | IL-17A | IL-23 | VEGF | G-CSF | IL-17A | IL-23 |
| D | IL-6 | GM-CSF | IL-2 | TPO | IL-6 | GM-CSF | IL-2 | TPO | IL-6 | GM-CSF | IL-2 | TPO |
| E | FGFb | MCP-1 | IL-4 | IP-10 | FGFb | MCP-1 | IL-4 | IP-10 | FGFb | MCP-1 | IL-4 | IP-10 |
| F | IFNy | MIP-1a | IL-10 | MCP-2 | IFNy | MIP-1a | IL-10 | MCP-2 | IFNy | MIP-1a | IL-10 | MCP-2 |
| G | EGF | SCF | Resistin | IL-33 | EGF | SCF | Resistin | IL-33 | EGF | SCF | Resistin | IL-33 |
| H | Leptin | Rantes | IL-12 | Blank | Leptin | Rantes | IL-12 | Blank | Leptin | Rantes | IL-12 | Blank |
Principle
The 96-well white plate is divided into 3 sections, and each section has 4 strips for one sample. In each section, 24 of specific cytokine capture antibodies are coated on 32 wells respectively, and one well without coating any antibody is used as a blank well. The sample, such as cell culture supernatants, cell lysates, tissue homogenates, serum, or plasma samples is incubated with cytokine ELISA plate, and the captured cytokine proteins are subsequently detected with a cocktail of biotinylated detection antibodies. The test sample is allowed to react with pairs of two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The plate is further detected with HRP luminescent substrate. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The level of expression for each specific cytokine is directly proportional to the luminescent intensity.
