Description(s):

Granulocyte colony-stimulating factor (G-CSF) is a colony-stimulating factor hormone. It is produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells and to stimulate the bone marrow to release them into the blood. It also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils, as well as monocytes.  G-CSF influences monocyte functions in an anti-inflammatory way.  The stimulation of monocytes with G-CSF results in an attenuation of LPS-induced release of IL-1, TNF-a, IL-12 and IL-18.

Detection Range:
- 16 - 1000 pg/mL 

Sensitivity:
- 8 pg/mL

Principle

Mouse Cytokine ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes rabbit anti-mouse antibodies for immobilization on the microtiter wells and rabbit anti-mouse antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The   concentration of G-CSF is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. 
 

 

 

Data

 

 

 

Literature

View user manual

Citations

VEGF optimizes the formation of tissue-engineered small intestine. Jamil A Matthews​‌1, Frédéric G Sala​‌1, Allison L Speer​‌1, David Warburton​‌1 & Tracy C Grikscheit​‌.  Regen Med. 2011 Sep;6(5):559-67.
 
Mesenchymal-Specific Inhibition of Vascular Endothelial Growth Factor (VEGF) Attenuates Growth in Neonatal Mice. Matthews JA, Sala FG, Speer AL, Li Y, Warburton D, Grikscheit TC. J Surg Res. 2012 Jan;172(1):40-7. Epub 2011 May 17. doi: 10.1016/j.jss.2011.04.043. Epub 2011 May 17.

Decreased plasma cytokines associate with low platelet counts in aplastic anemia and immune thrombocytopenic purpura. Feng X, Scheinberg P, Samsel L, Rios O, Chen J, McCoy JP Jr, Ghanima W, Bussel JB, Young NS.  J Thromb Haemost. 2012 Aug;10(8):1616-23.