Description(s):

Interleukin-6 (IL-6) is a multifunctional cytokine that regulates the immune response, hematopoiesis, the acute phase response, and inflammation. Deregulation of IL-6 production is implicated in the pathology of several disease processes.  Its levels are observed in several diseases, including rheumatoid arthritis (RA).  IL-6 plays roles in both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response.  In addition, like TNFa, IL-6 is another adipocyte secretory product that may be involved in insulin resistance.  IL-6 is a cytokine secreted by many cells, including adipocytes and adipose stromal cells.  IL-6 secretion is increased in the adipocytes of obese subjects and may be important either as a circulating hormone or as a local regulator of insulin action. IL-6 has been implicated in the development of insulin resistance and type 2 diabetes in obese individuals. Like TNF, IL-6 inhibits the expression of LPL, but, unlike TNF, IL-6 does not stimulate lipolysis.

Detection Range:
- 16 - 1000 pg/mL 

Sensitivity:
- 4 pg/mL

Principle

Mouse Cytokine ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes rabbit anti-mouse antibodies for immobilization on the microtiter wells and rabbit anti-mouse antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The   concentration of IL-6 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. 
 

 

 

Data

 

 

 

Literature

View user manual

Citations

VEGF optimizes the formation of tissue-engineered small intestine. Jamil A Matthews​‌1, Frédéric G Sala​‌1, Allison L Speer​‌1, David Warburton​‌1 & Tracy C Grikscheit​‌.  Regen Med. 2011 Sep;6(5):559-67.
 
Mesenchymal-Specific Inhibition of Vascular Endothelial Growth Factor (VEGF) Attenuates Growth in Neonatal Mice. Matthews JA, Sala FG, Speer AL, Li Y, Warburton D, Grikscheit TC. J Surg Res. 2012 Jan;172(1):40-7. Epub 2011 May 17. doi: 10.1016/j.jss.2011.04.043. Epub 2011 May 17.

Decreased plasma cytokines associate with low platelet counts in aplastic anemia and immune thrombocytopenic purpura. Feng X, Scheinberg P, Samsel L, Rios O, Chen J, McCoy JP Jr, Ghanima W, Bussel JB, Young NS.  J Thromb Haemost. 2012 Aug;10(8):1616-23.