Characterization
Description(s):Inflammation is a physiological process in response to tissue damage resulting from microbial pathogen infection, chemical irritation, and/or wounding. During both acute and chronic inflammatory processes, various types of leukocytes, lymphocytes, and other inflammatory cells are activated and attracted to the inflamed site by a signaling network involving a great number of growth factors, cytokines, and chemokines.
Signosis has developed a plate-based array for profiling 20+ inflammation-regulated genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.
Applicable Grid:
List of Applicable Genes
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | Eotaxin(Ccl11) | IL-6 | IL-14(Txlna) | Eotaxin(Ccl11) | IL-6 | IL-14(Txlna) | Eotaxin(Ccl11) | IL-6 | IL-14(Txlna) | Eotaxin(Ccl11) | IL-6 | IL-14(Txlna) |
| B | GCSF | IL-7 | IL-15 | GCSF | IL-7 | IL-15 | GCSF | IL-7 | IL-15 | GCSF | IL-7 | IL-15 |
| C | GMCSF | 18S rRNA | IL-16 | GMCSF | 18S rRNA | IL-16 | GMCSF | 18S rRNA | IL-16 | GMCSF | 18S rRNA | IL-16 |
| D | IL-1 | IL-9 | IL-17 | IL-1 | IL-9 | IL-17 | IL-1 | IL-9 | IL-17 | IL-1 | IL-9 | IL-17 |
| E | IL-2 | IL-10 | TGFa | IL-2 | IL-10 | TGFa | IL-2 | IL-10 | TGFa | IL-2 | IL-10 | TGFa |
| F | IL-3 | IL-11 | TGFb | IL-3 | IL-11 | TGFb | IL-3 | IL-11 | TGFb | IL-3 | IL-11 | TGFb |
| G | IL-4 | IL-12 | TNFa | IL-4 | IL-12 | TNFa | IL-4 | IL-12 | TNFa | IL-4 | IL-12 | TNFa |
| H | IL-5 | IL-13 | Blank | IL-5 | IL-13 | Blank | IL-5 | IL-13 | Blank | IL-5 | IL-13 | Blank |
Principle
Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.
Data
Mouse inflammaion cDNA plate array analysis. NIH3T3 cells were treated with (red bars) or without (light blue) 10ng/ml TNF for 90 min. Cells were collected for profiling 23 genes with the cDNA plate array assay.
