Description(s):

*New Product*

Insulin is a vital hormone secreted by the pancreas.  It is best known for its ability to control glucose levels in the blood stream; however it can also affect cellular activities such as DNA replication; protein and lipid synthesis; and the uptake of glucose, amino acids, and potassium.  The severity of diseases related to insulin disruption, such as Diabetes mellitus, demonstrates just how important this molecule is to the body.  The ability to simultaneously and quantifiably compare insulin level in many samples can improve our understanding of a multitude of cellular processes and disorders. Signosis’s Insulin ELISA kits allows you to simultaneously analyze insulin. Each well is coated with a specific capture antibody to detect insulin in various sample types. The expression levels of these cytokines can be quantitatively compared between samples.

Benefits:
- Increase in Sensitivity
- Detect Various Sample Types Including Serum, Cell Lysate, and Media Sample
- Quantitative Analysis from Standard Curve
- Quick Hands-On Assay Time

Detection Range:
-16 - 1000 pmol/L 
( 3 - 165 µIU/mL )

Sensitivity:
- 10 pmol/L

Principle

Mouse Cytokine ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes rabbit anti-mouse antibodies for immobilization on the microtiter wells and rabbit anti-mouse antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The   concentration of Leptin is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. 
 

 

 

 

Data

Literature

View user manual

 

Citations

VEGF optimizes the formation of tissue-engineered small intestine. Jamil A Matthews​‌1, Frédéric G Sala​‌1, Allison L Speer​‌1, David Warburton​‌1 & Tracy C Grikscheit​‌.  Regen Med. 2011 Sep;6(5):559-67.
 
Mesenchymal-Specific Inhibition of Vascular Endothelial Growth Factor (VEGF) Attenuates Growth in Neonatal Mice. Matthews JA, Sala FG, Speer AL, Li Y, Warburton D, Grikscheit TC. J Surg Res. 2012 Jan;172(1):40-7. Epub 2011 May 17. doi: 10.1016/j.jss.2011.04.043. Epub 2011 May 17.

Decreased plasma cytokines associate with low platelet counts in aplastic anemia and immune thrombocytopenic purpura. Feng X, Scheinberg P, Samsel L, Rios O, Chen J, McCoy JP Jr, Ghanima W, Bussel JB, Young NS.  J Thromb Haemost. 2012 Aug;10(8):1616-23.