Description(s):

Mouse leptin plays a key role in regulating energy intake and energy expenditure, including the regulation (decrease) of appetite and (increase) of metabolism.  Leptin is produced by adipose tissue and the level of circulating leptin is directly proportional to the total amount of fat in the body. Once leptin has bound to the Ob-Rb receptor, it activates Stat3, which is phosphorylated and travels to the nucleus where it mediates gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids, responsible—among their many other functions—for increasing appetite. There are other intracellular pathways activated by leptin, but less is known about how they function in this system. Although leptin is a circulating signal that reduces appetite, in general, obese people who are resistant to the effects of leptin have an unusually high circulating concentration of leptin, in the same way that people with type 2 diabetes are resistant to the effects of insulin.  Leptin is also found to stimulate endothelial cell proliferation and angiogenesis.

 

Detection Range:
- 16 - 1000 pg/mL 

Sensitivity:
- 8 pg/mL

 

 

Principle

Mouse Cytokine ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes rabbit anti-mouse antibodies for immobilization on the microtiter wells and rabbit anti-mouse antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The   concentration of Leptin is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. 
 

 

 

 

Data

 

 

 

Literature

View user manual

 

Citations

VEGF optimizes the formation of tissue-engineered small intestine. Jamil A Matthews​‌1, Frédéric G Sala​‌1, Allison L Speer​‌1, David Warburton​‌1 & Tracy C Grikscheit​‌.  Regen Med. 2011 Sep;6(5):559-67.
 
Mesenchymal-Specific Inhibition of Vascular Endothelial Growth Factor (VEGF) Attenuates Growth in Neonatal Mice. Matthews JA, Sala FG, Speer AL, Li Y, Warburton D, Grikscheit TC. J Surg Res. 2012 Jan;172(1):40-7. Epub 2011 May 17. doi: 10.1016/j.jss.2011.04.043. Epub 2011 May 17.

Decreased plasma cytokines associate with low platelet counts in aplastic anemia and immune thrombocytopenic purpura. Feng X, Scheinberg P, Samsel L, Rios O, Chen J, McCoy JP Jr, Ghanima W, Bussel JB, Young NS.  J Thromb Haemost. 2012 Aug;10(8):1616-23.