Description(s):
Monocyte chemotactic protein-1 (MCP-1), also called CCL2, is an inflammatory chemokine that plays important roles in recruiting monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. MCP-1 also involves in obesity and insulin resistance by the induction of an inflammatory response (macrophage infiltration) in fatty tissue. In addition, MPC1 has been found in the joints of people with rheumatoid arthritis where may serve to recruit macrophages and perpetuate the inflammation in the joints.
Detection Range:
- 16 - 1000 pg/mL
Sensitivity:
- 8 pg/mL
Principle
Mouse Cytokine ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes rabbit anti-mouse antibodies for immobilization on the microtiter wells and rabbit anti-mouse antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of MCP-1 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.
Data
Literature
View user manualCitations
VEGF optimizes the formation of tissue-engineered small intestine. Jamil A Matthews1, Frédéric G Sala1, Allison L Speer1, David Warburton1 & Tracy C Grikscheit. Regen Med. 2011 Sep;6(5):559-67.
Mesenchymal-Specific Inhibition of Vascular Endothelial Growth Factor (VEGF) Attenuates Growth in Neonatal Mice. Matthews JA, Sala FG, Speer AL, Li Y, Warburton D, Grikscheit TC. J Surg Res. 2012 Jan;172(1):40-7. Epub 2011 May 17. doi: 10.1016/j.jss.2011.04.043. Epub 2011 May 17.
Decreased plasma cytokines associate with low platelet counts in aplastic anemia and immune thrombocytopenic purpura. Feng X, Scheinberg P, Samsel L, Rios O, Chen J, McCoy JP Jr, Ghanima W, Bussel JB, Young NS. J Thromb Haemost. 2012 Aug;10(8):1616-23.