Description(s):
Troponin I is a component of the troponin protein complex, which regulates muscular contraction in thin myofilaments. It inhibits calcium-dependent muscle contraction by preventing myosin from binding to actin in relaxed muscle. Troponin I is a highly specific and sensitive biomarker of cardiac injury, and it can be easily detected by a blood test. The level of Troponin I increases after acute myocardial infraction and remains high for up to 14 days.
Principle
The wells of the plate are coated with capture antibody specific to troponin I. In this assay, the test sample initially reacts with the solid phase capture antibody, resulting in troponin I being bound to the well. The wells are then washed to remove unbound proteins, and biotin-linked antibodies are added to bind to the immobilized troponin I. After washing away the unbound antibodies, Streptavidin-HRP conjugate is added to form a complex with the antibody-bound troponin I. After incubation, the wells are washed to remove unbound Streptavidin-HRP conjugate. TMB substrate is then added and forms a blue color when the HRP-linked antibodies are detected. The reaction is then terminated with Stop Solution, which changes the color from blue to yellow. The troponin I concentration in each well is directly proportional to its color intensity and can be quantified by measuring its optical density at 450 nm (OD450) in a microplate reader.
