Characterization
Description(s):The Wnt signaling pathway is important to both embryonic development and tumorigenesis. ß-Catenin, the central component of the pathway, functions as a co-factor of the TCF/LEF family of transcription factors. Together, they activate transcription of Wnt target genes by binding to the promoters of downstream target genes involved in cell proliferation, survival, and migration. Signosis’ Wnt/ß-Catenin signaling pathway cDNA plate array can simultaneously measure the expression of 20+ genes that participate in the pathway and can compare the differences in the expression of these genes in different samples.
Signosis has developed a plate-based array for profiling 20+ Wnt-regulated genes. By using this assay, researchers are able to compare gene expression in up to three samples on a single microtiter plate.
Applicable Grid:
List of Applicable Genes
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | Axin-2 | Id2 | NOS2 | Axin-2 | Id2 | NOS2 | Axin-2 | Id2 | NOS2 | Axin-2 | Id2 | NOS2 |
| B | beta-actin | LEF1 | POU5F1 | beta-actin | LEF1 | POU5F1 | beta-actin | LEF1 | POU5F1 | beta-actin | LEF1 | POU5F1 |
| C | beta-catenin | Met | PPARd | beta-catenin | Met | PPARd | beta-catenin | Met | PPARd | beta-catenin | Met | PPARd |
| D | c-JUN | MMP1 | Sox9 | c-JUN | MMP1 | Sox9 | c-JUN | MMP1 | Sox9 | c-JUN | MMP1 | Sox9 |
| E | c-myc | MMP7 | Survivin | c-myc | MMP7 | Survivin | c-myc | MMP7 | Survivin | c-myc | MMP7 | Survivin |
| F | CyclinD | MMP9 | Tcf-1 | CyclinD | MMP9 | Tcf-1 | CyclinD | MMP9 | Tcf-1 | CyclinD | MMP9 | Tcf-1 |
| G | FosL1 | MMP26 | uPAR | FosL1 | MMP26 | uPAR | FosL1 | MMP26 | uPAR | FosL1 | MMP26 | uPAR |
| H | beta-actin | nanog | VEGF | beta-actin | nanog | VEGF | beta-actin | nanog | VEGF | beta-actin | nanog | VEGF |
Principle
Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.
Literature
View user manualCitations
1. Conditionally induced RAGE expression by proximal airway epithelial cells in transgenic mice causes lung inflammation.B Garrett Bodine, Brock G Bennion, Emma Leatham, Felix R Jimenez, Alex J Wright, Zac R Jergensen,
Connor J Erickson, Cameron M Jones, Jeff P Johnson, Steven M Knapp and Paul R Reynolds . Respiratory Research, 2014 (Published online)
2. Ablation of Doublecortin-Like Kinase 1 in the Colonic Epithelium Exacerbates Dextran Sulfate Sodium-Induced Colitis. D Qu, N Weygant, R May, P Chandrakesan. Plos One Published: August 18, 2015, DOI: 10.1371/journal.pone.0134212
