Characterization

Description(s):

NF-kappaB (NFkB) proteins comprise a family of eukaryotic transcription factors that are involved in the control of a large number of cellular and organismal processes. In addition, these transcription factors are associated with many diseases including cancer and arthritis. NFkB commonly refers specifically to a p50-RelA(p65) heterodimer, which is the major Rel/NF-kB complex in most cells. P65-p65 and p50-p50 heterodimers have been demonstrated to bind on DNA as well.  NF-kB is present as a latent, inactive, IkB-bound complex in the cytoplasm.  When a cell receives any of a multitude of extracellular signals, NF-kB rapidly enters the nucleus and activates gene expression. Signosis has developed the NFkB-p65 and NFkB p50 ELISA kits for sensitive and specific analysis of the activities of NFkB in a high throughput way. The kit can be used for human, mouse and rat samples.

Applicable Grid:

NF-kB ELISA Kits Available:

NF-kB p65 ELISA Kit (Colorimetric) TE-0001

NF-kB p65 ELISA Kit (Chemiluminescence) TE-0002

NF-kB p50 ELISA Kit (Colorimetric) TE-0003

NF-kB p50 ELISA Kit (Chemiluminescence) TE-0004

 

Principle

TF ELISA kit is high sensitive and specific assay with a simple and optimized procedure. The 96-well (8X12 strip) clear plate is pre-immobilized with the TF consensus sequencing oligo. The activated TF in nuclear extract or the whole cell lysate is added in the well and binds to the oligo. The activated TF is detected with a specific antibody against the subunit and a HRP conjugated secondary antibody. The assay utilizes colorimetric detection method, which can be easily measured by spectrophotometry.

Data

Analysis of NFkB activation with NFkB p65 ELISA in TNF-Treated HeLa Cells.

2ug HeLa and HeLa-TNF treated nuclear extracts are subjected to analyze with NFkB p65 ELISA kit. 

Analysis of NFkB activation with NFkB p65 ELISA in p65 overexpressed HeGP2 cells.  HeLa and p65 overexpressed nuclear extracts are subjected to analyze with NFkB p65 ELISA kit. 

Literature

View user manual

Citations

Antioxidant resveratrol restores renal sodium transport regulation in SHR, Apurva A. Javkhedkar, Anees A. Banday. Physiological Reports Published 24 November 2015 Vol. 3 no. e12618 DOI: 10.14814/phy2.12618