Description(s):

NIH 3T3 mouse embryonic fibroblast cells were initiated from a cell line isolated from a NIH Swiss mouse embryo in 1962 at the New York University School of Medicine. The cell line has since become a standard fibroblast cell line. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation.

  

Cell Line Origin

Mouse embryonic fibroblast

Principle

   

Cell line name	NIH/3T3
Catalog number	PC-004
Species	Mouse
Tissue	Fibroblast
Description	Tissue: Fibroblast, Embryo
Morphology/property	Fibroblast/Adherent
Growth	Continuous culture, grown as monolayer
Culture condition	Complete growth medium: In 450mL of DMEM, add 50mL FBS (10% final) and 5mL Penicillin/Streptomycin (1% final).
Subculturing	Subcultivation Ratio: Subculture twice per week at 80% confluence or less.
	Medium Renewal: Twice per week
Hazard	Handle as Biosafety Level 1 containment
Storage Conditions	Liquid nitrogen
Freezing medium	Complete growth medium + 10% DMSO